iPSC Culture: What Every Researcher Needs to Know
Induced pluripotent stem cells (iPSCs) have become a mainstay in disease modeling and drug development, offering almost unlimited opportunities to study human biology in the lab. However, working...
Visualizing ATP in a Cell
Genetically encoded biosensors have become popular and powerful LIVE-cell reporters in recent years (see reference here). These biosensors can be incorporated into a variety of human inducible...
Mesenchymal Stem Cells from Different Sources
The remarkable propensity of mesenchymal stem cells (MSCs) for self-renewal, multi-lineage differentiation and immune-modulatory activity has attracted much attention within the cell therapy area,...
What are Current Good Manufacturing Practice (CGMP) Requirements?
Pharmaceutical quality is of vital importance. Imagine getting a prescription from your doctor for drug X. You go to the pharmacy to pick it up, grab a bottle of water and then take the recommended...
How to Detect, Avoid and Deal With Contaminated Cell Cultures
There is nothing more tragic than walking into your tissue culture room only to find a researcher curled up on the floor, clasping a flask, and wailing that their cells have been contaminated. It's...
10 Easy Steps To Thoroughly Clean A Tissue Culture Hood
Your tissue culture hood, cell culture hood, biosafety cabinet (or BSC), the hood-y thing in your tissue culture room or whatever you like to call it, is a precious tool that requires occasional...
Matrigel vs. Geltrex Matrices: what’s the difference?
Basement membrane products like Matrigel® and Geltrex™ act as a substrate or physical support for cultured cells helping to create more in vivo-like extracellular matrices. Basement membranes are...
Cells, Spheroids and Tissues: the Pros and Cons of 3 Popular Cancer Cell Models
Cancer cell and tissue models are adapted for use in a research setting allowing for the direct study of a complex set of diseases. As models improve, their use in research has proven pivotal to...