Oxygen Metabolism Biosensor Assay


Oxygen Metabolism Biosensor Assay

TempoO2™, a fluorescent biosensor, is incorporated into Tempo’s proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution: seconds to hours) in measuring real-time cellular metabolism and cytotoxicity. Analysis of cellular respiration directly reflects mitochondrial health and function. TempoO2™-Rapid cells serve as a control for all products containing the TempoO2™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.

For filter-based instruments, Excitation/Emission: 617nm/635nm.
For monochromator-based instruments, Excitation 590nm +/- 20nm; Emission 650nm +/- 20nm.


TempoRapid™ cells

TempoO₂™-Rapid, Rotenone treated

TempoO₂™-Rapid, OligomycinA treated

TempoO₂™-Rapid, ActinomycinA treated


TempoO2™ cells are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Cytotoxicity Assays

Cellular Metabolism Assays

Chemical compound or small molecules testing

Live cell imaging (time-course studies and drug treatments).

For low, medium, or high-throughput applications

Easily transfected cells to test gene-of-interest function(s).


~0.6×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Product Use: TempoO2™-Rapid cells are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

TempoO2™-Rapid SKU402

Frequently Requested Cell Lines
The following are disease cell models that we are able to create through TempoO2

Cell Line Name Histology Type
U2OS Bone carcinoma
HEK293 Immortalized Kidney Epithelial
SW 480 Colorectal adenocarcinoma
U87MG Brain cancer
Hep G2 Hepatocellular carcinoma
NIH3T3 Embroyonic fibroblast, murine
HT1080 Carcinoma (connective tissue)
HCT116 Colorectal cancer
MCF 7 Breast cancer
SW 1990 Pancreatic cancer
NCI-H2126 Non-Small cell cancer
HUVEC Umbilical vascular endothelium
HeLa Cervical epithelial adenocarcinoma
CG4 Glial cell line (from rat)
A-204 Muscle epithelial
A549 Lung epithelial cell line
BT-142 Oligoastrocytoma, suspension
NK-92 Peripheral blood, natural killer cells
CHO Chinese hamster ovarian cell line
NCI-H69 Lung tumor cell aggregate, suspension
ARPE-19 Retinal pigmented epithelial
Beta-TC-6 Beta cell (mouse)
BxPC-3 Pancreatic epithelial, adenocarcinoma
Jurkat cells Peripheral T lymphocyte, suspension
SW 780 Urinary bladder epithelial carcinoma
MeT-5A Mesothelium, from non-cancerous
NFKB reporters Email us
Mesenchymal stromal cells Email us
Chemokine Reporter cells Email us
cell line of your choice Email us


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