Tempo-iCardio™
Human iPSC-derived Cardiomyocytes
Tempo-iCardio™
Human iPSC-derived Cardiomyocytes
Cardiomyocytes are cardiac muscle cells. They are terminally differentiated and facilitate contractile forces (“beatings”) of the heart. Grown in vitro as a monolayer sheath, cardiomyocytes are connected by gap junction proteins that help synchronize contraction-relaxation cycles of the cardiomyocytes. Cardiomyocytes may be used in various in vitro or in vivo studies; transplantation into normal or diseased systems; cardiac toxicology studies; or cardiovascular developmental studies. Cardiomyocytes have a high mitochondrial density, which allows them to produce adenosine triphosphate (ATP) quickly, making them highly resistant to fatigue.
Tempo-iCardio™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, genetic-vector–free, and integration-free conditions. They are cultured as monolayers and are alive for >4 weeks under defined cell culture conditions. Tempo-iCardio™ express biomarkers (q/RT-PCR gene expression analysis): ACTC1, CSPR3, KCNQ1, MYBPC3, MYH7, MYL2, PKP2, PRKAG2, TNNI3, TNNT, TPMI, TTN. Tempo-iCardio™ biomarkers (immunocytochemistry analysis): TNNT2, E-Cadherin.
Applications
Tempo-iCardio™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
Cardiac muscle disease modeling (e.g., Fabry disease, primary arrhythmias, cardiomyopathies, Pompe disease)
Cardiac toxicity and safety modeling (e.g., calcium flux assays, hERG)
Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)
Cardiac muscle disease modeling (e.g., Fabry disease, primary arrhythmias, cardiomyopathies, Pompe disease)
Specifications
~1.0×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
Technology used: an in-house developed proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)
Post-Thaw Viability: >4 weeks in culture under standard conditions
Tempo-iCardio™ SKU1014