Human iPSC-derived Mesenchymal Stem Cells


Human iPSC-derived Mesenchymal Stem Cells

Mesenchymal stem cells (“MSCs”) are non-haematopoietic, multipotent stromal stem cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, myocytes, and adipocytes. Tempo-iMSCTM are human iPSC-derived mesenchymal stem cells. Tempo-iMSCTM express cell surface markers such as CD73, CD90, CD166, and CD105, in addition to Oct4 and Nanog. This expression profile is predictive of the long-term proliferative capacity of cultured human MSCs (see reference: here). MSCs have immunomodulatory features, secrete cytokines and immune-receptors which regulate the microenvironment in the host tissue and they have multi-lineage potentials for a variety of cell types. MSCs can be used in a variety of screening applications, from screening biologics and small molecules to siRNAs. Due to MSCs’ ability to self-renew (= growth without differentiation), they are easily scalable for running large-scale assays.

NEW! For scientists working on exosomes, please email us for conditional media for exosome extractions and biomarker validations.

NEW! For scientists working on CD34+ HSCs, please email us regarding Tempo-iHSCTM (SKU214; Custom Requests Only).

Tempo-iMSC™ Immunocytochemistry: anti-human-Nanog (CST)

Tempo-iMSC™ Immunocytochemistry: anti-human-Oct4 (CST)

Tempo-iMSC™ RT-PCR data (left-to-right): CD90/Thy1, CD105, CD166, Oct4, Nanog

Tempo-iMSC™, monolayer, 9 days post-thaw

Tempo-iMSC™, monolayer, 10 days post-thaw


Tempo-iMSC™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Phenotypic Assays

High Content Imaging

Biomarker Discovery

Cytotoxicity Assays

Target Validation

Lead Optimization

Investigative Toxicology

Nonclinical Efficacy Evalutions

Live-cell Imaging

2D & 3D Cell Culture

Biomarker Authentication


~1.25 x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent monolayer

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: in-house developed proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.

Starting Materials: human dermal/fibroblast cells

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iMSC™ SKU209


Cell of the Month: Mast Cells

Cell of the Month: Mast Cells

Mast cells are tissue-resident immune cells derived from the myeloid lineage, and along with basophils, eosinophils and neutrophils, they belong to the granulocyte family of white blood cells.  First discovered and named almost 200 years ago by...

read more
Cell of the Month: Pericytes

Cell of the Month: Pericytes

It’s a fibroblast-like cell adorned with long cytoplasmic processes that wrap around the endothelial cells in blood vessels, it controls blood flow through the blood vessels, and it is essential for normal brain function and development. Which cell...

read more