Human iPSC-derived Dopaminergic Neurons


Human iPSC-derived Dopaminergic Neurons

Dopaminergic neurons belong to one of the most important neuronal subtypes in the human central nervous system (CNS). In the CNS, these neurons are primarily responsible for transmitting dopamine and they form networks that are responsible for a variety of functions in the substantia nigra, ventral tegmental area, hypothalamus, and zona incerta. They are implicated in many disabling neurological disorders, including Parkinson’s, drug addiction, psychosis, and Attention Deficit Hyperactivity Disorder (ADHD).

Tempo’s iDopaNer™: human dopaminergic neurons are derived from human dermal cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iDopaNer™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta3-Tuj (neuronal), tyrosine hydroxylase (TH), and dopamine transporter (DAT). iDopaNer™ can be used under maturation conditions, co-culture conditions (with astrocytes or additional neuronal subtypes), and short-term functional characterization studies


iDopaNer™ ICC data with anti-TH

iDopaNer™ Post-Thaw Day4

iDopaNer™ ICC data with anti-TH

iDopaNer™ ICC data with anti-beta-Tuj3 (neuronal marker)


Tempo-iDopaNer™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Phenotypic Assays

High Content Imaging

Biomarker Discovery

Cytotoxicity Assays

Target Validation

Lead Optimization

Investigative Toxicology

Nonclinical Efficacy Evalutions

Live-cell Imaging

2D & 3D Cell Culture

Biomarker Authentication


Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

Specifications: ~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iDopaNer™ SKU206