Tempo-iNStem ™

Human iPSC-derived Neural Progenitor Cells

Tempo-iNStem™

Human iPSC-derived Neural Progenitor Cells

Neural stem cells are self-renewing, multipotent cells that can generate neurons and glial-specific cellular lineages. Primarily, neural stem cells differentiate into neurons, astrocytes, and oligodendrocytes. Neural progenitor cells are adult stem cells committed to their neural and glial lineages.

Tempo-iNStem™ cells are induced neural progenitor cells reprogrammed from human fibroblasts or blood cells using proprietary foot-print-free stem cell technologies. iNStem™ cells are cryopreserved at low passage numbers. Each vial will maintain 5 subsequent passages and expansions without spontaneous differentiations. Cells have been tested to be able to differentiate into multiple neural and glial subtypes. Each vial contains >1 x 106 cells. iNStem™  cells are characterized via standard morphological examinations and via immunocytochemistry methods against known marker proteins (such as Vimentin).

 Tempo’s iNStem™ are human iPSC-derived neural progenitor cells. They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iNStem™  cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We have validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro serum-free/integration-free reprogramming conditions.

iNStem™ induced neural progenitor cells

iNStem™ induced neural progenitor cells. Immunocytochemistry with anti-Vimentin antibodies.

Applications

Tempo-iNStem™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Research and development of cell therapies in animal models


Disease modeling


Phenotypic Assays (e.g., induction of lineage-specific marker proteins)


Neurotoxicity testing


Studying neurogenesis, neuronal differentiation and function, and neural network formation

Specifications

~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iNStem™ SKU100

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