Mitochondrial Biosensor Assay

TempoMito™-U2OS Cell Line

U2-OS cell line, originally known as the 2T cell line, is an immortalized human cancer cell line. It is widely used in research and drug development, as it offers a variety of application potentials.

TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). U2-OS stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter.

Culture Medium: McCoy′s 5a medium with 1.5 mM Glutamine or DMEM with 2mM L-Glutamine; 10% Fetal Bovine Serum (FBS). Subculture Routine: Split sub-confluent using 0.025% trypsin/EDTA solution. Allow the flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. Passage ratio: 1-to-6

TempoMito™ Mitochondrial Biosensor Assay using Human Astrocytes

TempoMito™-iAstro cells contain mitochondrial biosensors in human iPS-derived astrocytes. Disruption of mitochondrial functions in human astrocytes has been implicated in Alzheimer’s disorder and ischemia. Tempo’s biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening.

TempoMito™-iAstro: Mitochondrial Calcium Biosensor Assay using human Astrocytes

TempoMito™-iAstro are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary astrocytes induction condition. TempoMito™-iAstro cells are plated as monolayers in culture and express markers such as GFAP. TempoMito™ biosensor is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoMito™ Mitochondrial Biosensor Assay using Human Neural Progenitors

TempoMito™-iNStem cells comprise mitochondrial biosensors incorporated into human iPS-derived neural progenitor cells. Mitochondrial dysfunctions have been implicated in human neurological and psychiatric disorders, and neural progenitor cells have been used as a cellular model. Tempo’s biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening.

TempoMito™-iNStem;: Mitochondrial Calcium Biosensor Assay using human Neural Stem Cells. They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. TempoMito™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We’ve validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoMito biosensor is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoMito™ Mitochondrial Biosensor Assay using Immortalized Human Neuroepithelial Cells

TempoMito™ mitochondrial calcium biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range. It is incorporated into Tempo’s proprietary immortalized human neuroepithelial cell line, TempoRapidTM. TempoMito™ control cells serve as a control for all products containing the TempoMito™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.