Tempo-iOligo™

Human iPSC-derived Oligodendrocyte Progenitor Cells (OPCs)

OverviewApplicationSpecificationsResources

Tempo-iOligo™

Human iPSC-derived Oligodendrocyte Progenitor Cells (OPCs)

Oligodendrocytes belong to the family of glial cells in the human central and peripheral nervous system. They are multifunctional cells and are implicated in many disabling neurological disorders, including adult-onset demyelinating disorders, as well as leukodystrophies and cerebral palsy.

Tempo’s iOligo™ are induced oligodendrocyte progenitors reprogrammed from human fibroblasts cells using a proprietary reprogramming technology. iOligo™ cells are cryopreserved at low passage numbers. Each vial contains ~1.05 x 106 cells. iOligo™ cells are characterized via standard morphological examinations and via immunocytochemistry methods against known marker proteins (such as O4, PDGFalphaR, NG2, and CNPase) and can be induced to express MBP upon T3 hormone induction

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iOligo™ (O4 marker, Green)

iOligo™ ICC staining, anti-human-PDGFalphaR antibody

iOligo™ human oligodendrocytes and progenitor cells, immediate induction by T3, day5

iOligo™ T3-induced for 25 days, anti-human-MBP (ICC)

iOligo™ without induction, ICC staining, anti-human-O4

iOligo™ without induction, ICC staining, anti-human-O4

iOligo™ post-thaw 24 hours

Applications

Tempo-iOligo™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Phenotypic Assays

High Content Imaging

Biomarker Discovery

Cytotoxicity Assays

Target Validation

Lead Optimization

Investigative Toxicology

Nonclinical Efficacy Evalutions

Live-cell Imaging

2D & 3D Cell Culture

Biomarker Authentication

Tempo’s “Build an organoid” is easy to scale!

Ready in DAYS NOT WEEKS, and simplifies assay and data analysis.

CNS Organoid

CNS Organoid

iCort, iAstro,
iOligo, iMG

Customize Neural : Glia
ratio to 6:1, 3:1, 4:1

10-14 Days Controlled Assembly of Organoids with Custom Ratio

Easily Scalable

Specifications

~1.05×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iOligo™ SKU102