Tempo-iOligo™

Human iPSC-derived Oligodendrocyte Progenitor Cells (OPCs)

Tempo-iOligo™

Human iPSC-derived Oligodendrocyte Progenitor Cells (OPCs)

Oligodendrocytes belong to the family of glial cells in the human central and peripheral nervous system. They are multifunctional cells and are implicated in many disabling neurological disorders, including adult-onset demyelinating disorders, as well as leukodystrophies and cerebral palsy.

Tempo’s iOligo™ are induced oligodendrocyte progenitors reprogrammed from human fibroblasts cells using a proprietary reprogramming technology. iOligo™ cells are cryopreserved at low passage numbers. Each vial contains ~1.05 x 106 cells. iOligo™ cells are characterized via standard morphological examinations and via immunocytochemistry methods against known marker proteins (such as O4, PDGFalphaR, NG2, and CNPase) and can be induced to express MBP upon T3 hormone induction

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iOligo™ (O4 marker, Green)

iOligo™ ICC staining, anti-human-PDGFalphaR antibody

iOligo™ human oligodendrocytes and progenitor cells, immediate induction by T3, day5

iOligo™ T3-induced for 25 days, anti-human-MBP (ICC)

iOligo™ without induction, ICC staining, anti-human-O4

iOligo™ without induction, ICC staining, anti-human-O4

iOligo™ post-thaw 24 hours

Applications

Tempo-iOligo™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Research and Development of cell therapies in animal models


Disease modeling


Phenotypic Assays (e.g., expression of differentiation markers)


Toxicity testing


Studying oligodendrocyte differentiation, function, and myelination

Specifications

~1.05×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iOligo™ SKU102

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