TempoATP™
ATP Biosensor Assay
TempoATP™
ATP Biosensor Assay
TempoATP™, a fluorescent biosensor, is incorporated into Tempo’s proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function.
TempoATP™ + TempoRapid™
TempoATP™ ATP Biosensor Assay using Immortalized Human Neuroepithelial Cells
TempoATP™-Rapid cells serve as a control for all products containing the TempoATP™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.
For filter-based instruments, Excitation/Emission: 619nm/635nm (Red) or Excitation/Emission: 517nm/535nm (Green)
For monochromator-based instruments, Excitation 590nm +/- 25nm; Emission 655nm +/- 20nm.
Applications
TempoATP™ incorporated cells are intended for research and development, compound discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
Cytotoxicity Assays
Cellular Metabolism Assays
Chemical compound or small molecules testing
Live cell imaging (time-course studies and drug treatments).
For low, medium, or high-throughput applications
Easily transfected cells to test gene-of-interest function(s).
Specifications
~0.6×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming.
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)
Product Use: TempoATP™-Rapid cells are intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
TempoATP™-Rapid SKU401
Frequently Requested Cell Lines
The following are disease cell models that we are able to create through TempoATP™
| Cell Line Name | Histology Type | |
| U2OS | Bone carcinoma | |
| HEK293 | Immortalized Kidney Epithelial | |
| SW 480 | Colorectal adenocarcinoma | |
| U87MG | Brain cancer | |
| Hep G2 | Hepatocellular carcinoma | |
| NIH3T3 | Embroyonic fibroblast, murine | |
| HT1080 | Carcinoma (connective tissue) | |
| HCT116 | Colorectal cancer | |
| MCF 7 | Breast cancer | |
| SW 1990 | Pancreatic cancer | |
| NCI-H2126 | Non-Small cell cancer | |
| HUVEC | Umbilical vascular endothelium | |
| HeLa | Cervical epithelial adenocarcinoma | |
| CG4 | Glial cell line (from rat) | |
| A-204 | Muscle epithelial | |
| A549 | Lung epithelial cell line | |
| BT-142 | Oligoastrocytoma, suspension | |
| NK-92 | Peripheral blood, natural killer cells | |
| CHO | Chinese hamster ovarian cell line | |
| NCI-H69 | Lung tumor cell aggregate, suspension | |
| ARPE-19 | Retinal pigmented epithelial | |
| Beta-TC-6 | Beta cell (mouse) | |
| BxPC-3 | Pancreatic epithelial, adenocarcinoma | |
| Jurkat cells | Peripheral T lymphocyte, suspension | |
| SW 780 | Urinary bladder epithelial carcinoma | |
| MeT-5A | Mesothelium, from non-cancerous | |
| NFKB reporters | Email us | |
| Mesenchymal stromal cells | Email us | |
| Chemokine Reporter cells | Email us | |
| cell line of your choice | Email us |
Citation
References
- Martin LJ. Prog Mol Biol Transl Sci. 2012;107:355-415.
- Martin LJ. Pharmaceuticals (Basel). 2010;3(4):839-915.
- Chen K, Northington FJ, Martin LJ. Brain Struct Funct. 2010 Mar;214(2-3):219-34.
- Palomo GM, Manfredi G. Brain Res. 2015 May 14;1607:36-46.
- Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y. Toxicol Sci. 2008 Jun;103(2):335-45.
- Dykens JA, Will Y. Drug Discov today. 2007 Sep; 12 (17-18): 777-85.