TempoATP™

ATP Biosensor Assay

TempoATP™

ATP Biosensor Assay

TempoATP™, a fluorescent biosensor, is incorporated into Tempo’s proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function.

TempoATP™ + TempoRapid™

TempoATP™ ATP Biosensor Assay using Immortalized Human Neuroepithelial Cells

TempoATP™-Rapid cells serve as a control for all products containing the TempoATP™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.

For filter-based instruments, Excitation/Emission: 619nm/635nm (Red) or Excitation/Emission: 517nm/535nm (Green)
For monochromator-based instruments, Excitation 590nm +/- 25nm; Emission 655nm +/- 20nm.

TempoRapid™ Cells

TempoATP™ incorporated cells, SnapShot

TempoATP™ incorporated cells, SnapShot

TempoATP™ cells, OligomycinA treated

TempoATP™ cells, Rotenone treated

TempoATP™ biosensor

Applications

TempoATP™ incorporated cells are intended for research and development, compound discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Cytotoxicity Assays


Cellular Metabolism Assays


Chemical compound or small molecules testing


Live cell imaging (time-course studies and drug treatments).


For low, medium, or high-throughput applications


Easily transfected cells to test gene-of-interest function(s).

Specifications

~0.6×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Product Use: TempoATP™-Rapid cells are intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

TempoATP™-Rapid SKU401

Frequently Requested Cell Lines
The following are disease cell models that we are able to create through TempoATP™

Cell Line Name Histology Type
U2OS Bone carcinoma
HEK293 Immortalized Kidney Epithelial
SW 480 Colorectal adenocarcinoma
U87MG Brain cancer
Hep G2 Hepatocellular carcinoma
NIH3T3 Embroyonic fibroblast, murine
HT1080 Carcinoma (connective tissue)
HCT116 Colorectal cancer
MCF 7 Breast cancer
SW 1990 Pancreatic cancer
NCI-H2126 Non-Small cell cancer
HUVEC Umbilical vascular endothelium
HeLa Cervical epithelial adenocarcinoma
CG4 Glial cell line (from rat)
A-204 Muscle epithelial
A549 Lung epithelial cell line
BT-142 Oligoastrocytoma, suspension
NK-92 Peripheral blood, natural killer cells
CHO Chinese hamster ovarian cell line
NCI-H69 Lung tumor cell aggregate, suspension
ARPE-19 Retinal pigmented epithelial
Beta-TC-6 Beta cell (mouse)
BxPC-3 Pancreatic epithelial, adenocarcinoma
Jurkat cells Peripheral T lymphocyte, suspension
SW 780 Urinary bladder epithelial carcinoma
MeT-5A Mesothelium, from non-cancerous
NFKB reporters Email us
Mesenchymal stromal cells Email us
Chemokine Reporter cells Email us
cell line of your choice Email us

Citation

References

  • Martin LJ. Prog Mol Biol Transl Sci. 2012;107:355-415.
  • Martin LJ. Pharmaceuticals (Basel). 2010;3(4):839-915.
  • Chen K, Northington FJ, Martin LJ. Brain Struct Funct. 2010 Mar;214(2-3):219-34.
  • Palomo GM, Manfredi G. Brain Res. 2015 May 14;1607:36-46.
  • Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y. Toxicol Sci. 2008 Jun;103(2):335-45.
  • Dykens JA, Will Y. Drug Discov today. 2007 Sep; 12 (17-18): 777-85.
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