TempoVOL™

Cationic Voltage Biosensor Assay

TempoVOL™

Cationic Voltage Biosensor Assay

TempoVol™-iCort: cationic voltage biosensor assay using human iPS-derived . They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction and maturation conditions. TempoVol™-iCort cells are polarized structures when plated as a monolayer in culture and express markers such as MAP2 and beta-Tuj. We’ve validated iCort™ cells to be cationic-conducting neurons when exposed to solutions with fluctuating concentrations of sodium, calcium, potassium, magnesium, and protons/H+. TempoVol™ biosensor is introduced to the cells using proprietary methods and is expressed in the in abundance in the cell.

TempoVol ™ + Tempo-iCort ™

TempoVol™ Cationic Voltage Biosensor Assay using Human Cortical Neurons

TempoVol™-iCort: cationic voltage biosensor assay using human iPS-derived . They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction and maturation conditions. TempoVol™-iCort cells are polarized structures when plated as a monolayer in culture and express markers such as MAP2 and beta-Tuj. We’ve validated iCort™ cells to be cationic-conducting neurons when exposed to solutions with fluctuating concentrations of sodium, calcium, potassium, magnesium, and protons/H+. TempoVol™ biosensor is introduced to the cells using proprietary methods and is expressed in the in abundance in the cell.

TempoVol ™ + Tempo iNStem™

TempoVol™ Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells

TempoVol™ cationic voltage biosensors are incorporated into human neural progenitor cells. They respond to intracellular ionic changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium/potassium/proton/sodium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission: 610nm/625nm) and is incorporated into Tempo’s iPSC-derived neural progenitor cells, iNStem. iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We’ve validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoVol™ biosensor is introduced to the cells using proprietary liposome mediated methods.

TempoVol ™ + TempoRapid™

TempoVol™ Cationic Voltage Biosensor Assay using Immortalized Human Neuroepithelial Cells

TempoVol™ cationic voltage biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission: 610nm/625nm). It is incorporated into Tempo’s proprietary immortalized human neuroepithelial cell line, TempoRapidTM. TempoVol™ control cells serve as a control for all products containing the TempoVol™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

iPS-derived cortical neurons; ICC using anti-beta-Tuj

Tempo's iNStem, Vimentin biomarker (green)

TempoVOL™-iCort kinetic responses to H+ and Ca2+

TempoVOL™ in TempoRapid cells

TempoVOL™-iCort kinetic responses to H+ and Ca2+

TempoVOL™ responses

TempoVOL™ functions in HEK293t cells

Applications

TempoVol™-iNStem are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.


Neurotoxicity testing for reporting cellular cationic fluctuations (such as calcium, protons/H+, potassium)


Phenotypic Assays


Chemical compound or small molecules testing and screening


Signal transduction pathway modulations


Ion Channel studies


Studying neural maturation, networking and function, and cations-mediated pathways


For low, medium, or high-throughput applications


Research and development on animal models for human diseases


Control cell line with biosensor incorporated (e.g., establishing baseline response levels, testing expression constructs)


Neurotoxicity testing for reporting cationic flux

Specifications

~2×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

TempoVol™-iCort SKU202
Specifications: ~1.05×10^6 cells per 1ml of freezing medium (vial)

TempoCal™-iNStem SKU121
Specifications: ~2×10^6 cells per 1ml of freezing medium (vial)

TempoCal™-Rapid SKU120
Specifications: ~1×10^6 cells per 1ml of freezing medium (vial)
Starting Materials: immortalized human cell line of neuro-epithelial origin. Cells should grow fairly well (doubling time < 36 h) without passage limitation in a conventional medium: DMEM or DMEM/F12 media with a maximum of 10% serum, 1x essential amino acids, Pen/Strep and selection antibiotics (such as G418, hygromycin or puromycin) at experimentally determined concentrations for each user’s experiment if a gene-of-interest is introduced with a selection marker.

 

 

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