Tempo-iKer™
Human iPSC-derived Keratinocytes
Tempo-iKer™
Human iPSC-derived Keratinocytes
Keratinocytes are the predominant cells in the epidermal layer of human skin. ~90% of the human epidermis is constituted of keratinocytes. The most salient function of keratinocytes is their ability to form a barrier against outside damages by bacteria, fungi, parasites, viruses, heat, dehydration, and ultraviolet (UV).
Tempo-iKer™: human keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, non-viral, nucleic-acids-free, induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iKer™ cells are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratins 14 and 18. iKer™ can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.
Applications
Tempo-iKer™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
Studies related to epidermal functions
Skin disease modeling
Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)
Toxicity testing
Skin models (2D or 3D)
Bioprinting of skin
Specifications
~1.25×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)
Tempo-iKer™ SKU131