Human iPSC-derived Keratinocytes


Human iPSC-derived Keratinocytes

Keratinocytes are the predominant cells in the epidermal layer of human skin. ~90% of the human epidermis is constituted of keratinocytes. The most salient function of keratinocytes is their ability to form a barrier against outside damages by bacteria, fungi, parasites, viruses, heat, dehydration, and ultraviolet (UV).

Tempo-iKer™: human keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, non-viral, nucleic-acids-free, induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iKer™ cells are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratins 14 and 18. iKer™ can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.

Tempo-iKer™, ICC using anti-human-CK18


Tempo-iKer™, ICC using anti-human-CK18

Tempo-iKer™, ICC using anti-human-CK18


Tempo-iKer™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Phenotypic Assays

High Content Imaging

Biomarker Discovery

Cytotoxicity Assays

Target Validation

Lead Optimization

Investigative Toxicology

Nonclinical Efficacy Evalutions

Live-cell Imaging

2D & 3D Cell Culture

Biomarker Authentication


 ~1.25×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iKer™ SKU131