Tempo-iKer™

Human iPSC-derived Keratinocytes

Tempo-iKer™

Human iPSC-derived Keratinocytes

Keratinocytes are the predominant cells in the epidermal layer of human skin. ~90% of the human epidermis is constituted of keratinocytes. The most salient function of keratinocytes is their ability to form a barrier against outside damages by bacteria, fungi, parasites, viruses, heat, dehydration, and ultraviolet (UV).

Tempo-iKer™: human keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, non-viral, nucleic-acids-free, induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iKer™ cells are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratins 14 and 18. iKer™ can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.

Tempo-iKer™, ICC using anti-human-CK18

Tempo-iKer™

Tempo-iKer™, ICC using anti-human-CK18

Tempo-iKer™, ICC using anti-human-CK18

Applications

Tempo-iKer™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Studies related to epidermal functions


Skin disease modeling


Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)


Toxicity testing


Skin models (2D or 3D)


Bioprinting of skin

Specifications

 ~1.25×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iKer™ SKU131

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