Human iPSC-derived Cortical Neurons


Human iPSC-derived Cortical Neurons

Cortical neurons belong to the family of diverse neurons in the human central nervous system (CNS). In the CNS, these neurons form networks that are responsible for associative and cognitive functions. There are excitatory projection neurons (PNs) and inhibitory interneurons (INs). They are are implicated in many disabling neurological disorders, including Alzheimer’s and Lofora diseases.

Tempo’s iCort™: human cortical neurons are derived from human dermal cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iCort™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta-3-Tuj, GAD65/67, VGLUT1/2, and Map2. iCort™ can be used under maturation conditions, co-culture conditions (with astrocytes), and short-term functional characterization studies.

Tempo-iCort™ ICC anti-Map2 (CST)

Tempo-iCort™ ICC anti-beta-3-Tuj (CST)

Tempo-iCort™ ICC anti-GAD65/67 (Millipore)

Tempo-iCort™ ICC anti-beta-3-Tuj (CST)

Tempo-iCort™ post-thaw image


Tempo-iCort™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Phenotypic Assays

High Content Imaging

Biomarker Discovery

Cytotoxicity Assays

Target Validation

Lead Optimization

Investigative Toxicology

Nonclinical Efficacy Evalutions

Live-cell Imaging

2D & 3D Cell Culture

Biomarker Authentication

Tempo’s “Build an organoid” is easy to scale!

Ready in DAYS NOT WEEKS, and simplifies assay and data analysis.

CNS Organoid

CNS Organoid

iCort, iAstro,
iOligo, iMG

Customize Neural : Glia
ratio to 6:1, 3:1, 4:1

10-14 Days Controlled Assembly of Organoids with Custom Ratio

Easily Scalable


~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iCort™ SKU201