Human iPSC-derived Motor Neurons
Motor neurons belong to one of the most specialized neuronal subtypes in the human central and peripheral nervous system. These neurons are primarily responsible for transmitting signals to effector cell types. Their cell bodies are found in the motor cortex, brainstem, or spinal cord. Their axons (fibers) project to effector cell types or tissue types, such as muscles and glands. Degeneration or dysfunction of motor neurons can lead to several devastating disorders, including amyotrophic lateral sclerosis (ALS or Lou Gerig’s), primary lateral sclerosis, progressive bulbar palsy, and other subtypes of motor neuron diseases.
Tempo’s iMotorNer™ cells are reprogrammed from human iPS-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free conditions. Motor neurons have synapses that transmit via cholinergic transmitters. These synapses require extremely fast inactivations of its transmitter, acetylcholine (Ach), by utilizing the enzyme, acetylcholinesterase (AchT). AchT hydrolyzes acetylcholine to acetate and choline, as a way of inactivation. Tempo’s iMotorNer™ cells are polarized structures when plated as a monolayer in culture and express biomarkers, acetylcholinesterase (AchT), beta-TUJ (neuron-specific), Isl-1 and HB9.
Tempo-iMotorNer™ anti-MAP2 antibody
Tempo-iMotorNer™ Post-Thaw, 24-hours
Tempo-iMotorNer™ anti-acetylcholinesterase antibody against human AchT protein
Tempo-iMotorNer™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
Disease modeling (e.g., ALS/Lou Gerig’s, Motor Neuron Disorders)
In-vitro assays using motor neurons
Phenotypic Screening (e.g., co-culture with muscle tubules)
Developmental biomarker and co-culture (with myotube) studies
~1.02×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
Technology used: an in-house developed proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.
QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)