Tempo-iAstro™

Human iPSC-derived Astrocytes

Tempo-iAstro™

Human iPSC-derived Astrocytes

Astrocytes, also known as Astroglia, belong to the family of glial cells in the human central and peripheral nervous system. They have been shown to have central roles in the maintenance of neuronal metabolism and neurotransmitter synthesis. They have been implicated in many human neurodegenerative disorders such as multiple sclerosis, amyotrophic lateral sclerosis (ALS), Alzheimer’s disorder, Huntington’s disease and Parkinson’s disease.

Tempo’s iAstro™: human iPSC-derived astrocytes are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iAstro cells are polarized structures when plated as a monolayer in culture and express astrocyte markers, GFAP, EAAT1, Aquaporin family genes, and S100beta, and they exhibit calcium wave activities (see figures shown above). There are two subtypes of human astrocytes: Type I and Type II. >90% of Tempo-iAstro™ astrocytes are Type II. If you need Type I astrocytes, please email Tempo Support.

iAstro™ human astrocytes, light transmission

iAstro™ human astrocytes, anti-GFAP antibody

iAstro™ human astrocytes, anti-S100beta antibody

iAstro™ human astrocytes, anti-S100beta antibody

Calcium imaging of human astrocytes

Applications

Tempo-iAstro™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Research and development of cell therapies in animal models


Disease modeling


Phenotypic Screening (e.g., cytokine secretion, expression of differentiation markers)


Toxicity testing


Studying astrocyte differentiation and function

Specifications

~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iAstro™ SKU101

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