Human iPSC-derived Astrocytes


Human iPSC-derived Astrocytes

Astrocytes, also known as Astroglia, belong to the family of glial cells in the human central and peripheral nervous system. They have been shown to have central roles in the maintenance of neuronal metabolism and neurotransmitter synthesis. They have been implicated in many human neurodegenerative disorders such as multiple sclerosis, amyotrophic lateral sclerosis (ALS), Alzheimer’s disorder, Huntington’s disease and Parkinson’s disease.

Tempo’s iAstro™: human iPSC-derived astrocytes are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iAstro cells are polarized structures when plated as a monolayer in culture and express astrocyte markers, GFAP, EAAT1, Aquaporin family genes, and S100beta, and they exhibit calcium wave activities (see figures shown above). There are two subtypes of human astrocytes: Type I and Type II. >90% of Tempo-iAstro™ astrocytes are Type II. If you need Type I astrocytes, please email Tempo Support.

iAstro™ human astrocytes, light transmission

iAstro™ human astrocytes, anti-GFAP antibody

iAstro™ human astrocytes, anti-S100beta antibody

iAstro™ human astrocytes, anti-S100beta antibody

Calcium imaging of human astrocytes


Tempo-iAstro™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Phenotypic Assays

High Content Imaging

Biomarker Discovery

Cytotoxicity Assays

Target Validation

Lead Optimization

Investigative Toxicology

Nonclinical Efficacy Evalutions

Live-cell Imaging

2D & 3D Cell Culture

Biomarker Authentication

Tempo’s “Build an organoid” is easy to scale!

Ready in DAYS NOT WEEKS, and simplifies assay and data analysis.

CNS Organoid

CNS Organoid

iCort, iAstro,
iOligo, iMG

Customize Neural : Glia
ratio to 6:1, 3:1, 4:1

10-14 Days Controlled Assembly of Organoids with Custom Ratio

Easily Scalable


~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iAstro™ SKU101