Tempo-iMG™

Human iPSC-derived Microglia

Tempo-iMG™

Human iPSC-derived Microglia

Tempo-iMG™ microglia cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming conditions. Tempo’s iMG™ microglia cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, A1F1(= IBA1), hP2RY-12, TREM2, hGPR34, CXCR3, P2RX4, P2RX7, TRPM7, and CD11b/c. They exhibit phagocytosis activities (shown via LIVE-cell phagocytosis assay using fluorescent beads). Microglia are different from macrophages in many significant ways: synaptic stripping, secreting cytotoxic molecules, removing waste molecules from neurons, and signaling robust calcium wave responses.

Tempo-iMG™ ICC, anti-human-CXCR3 antibody

Tempo-iMG™ ICC, anti-human-CD11b/c antibody

Tempo-iMG™ post-thaw image; QC image.

Tempo-iMG™ post-thaw image; QC image.

Tempo-iMG™ activated; QC image.

Tempo-iMG™ TempoCal™ biosensor; calcium imaging; fluorescent multi-plate reader

Applications

Tempo-iMG™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Disease modeling


Cytotoxicity evaluations


Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)


Preclinical validation studies


In vitro assays using microglial cells

Specifications

~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iMG™ SKU1001.1

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