Tempo-iMG™

Human iPSC-derived Microglia

OverviewApplicationSpecificationsResources

Tempo-iMG™

Human iPSC-derived Microglia

Tempo-iMG™ microglia cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming conditions. Tempo’s iMG™ microglia cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, A1F1(= IBA1), hP2RY-12, TREM2, hGPR34, CXCR3, P2RX4, P2RX7, TRPM7, and CD11b/c. They exhibit phagocytosis activities (shown via LIVE-cell phagocytosis assay using fluorescent beads). Microglia are different from macrophages in many significant ways: synaptic stripping, secreting cytotoxic molecules, removing waste molecules from neurons, and signaling robust calcium wave responses.

Tempo-iMG™ ICC, anti-human-CXCR3 antibody

Tempo-iMG™ ICC, anti-human-CD11b/c antibody

Tempo-iMG™ post-thaw image; QC image.

Tempo-iMG™ post-thaw image; QC image.

Tempo-iMG™ activated; QC image.

Tempo-iMG™ TempoCal™ biosensor; calcium imaging; fluorescent multi-plate reader

Applications

Tempo-iMG™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.


Disease modeling


Cytotoxicity evaluations


Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)


Preclinical validation studies


In vitro assays using microglial cells

Tempo’s “Build an organoid” is easy to scale!

Ready in DAYS NOT WEEKS, and simplifies assay and data analysis.

CNS Organoid

CNS Organoid

iCort, iAstro,
iOligo, iMG

Customize Neural : Glia
ratio to 6:1, 3:1, 4:1

10-14 Days Controlled Assembly of Organoids with Custom Ratio

Easily Scalable

Specifications

~1.0×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

Tempo-iMG™ SKU1001.1