Human iPSC-derived Brain Microvascular Endothelial Cells

Tempo-iBMEC™

Human iPSC-derived Brain Microvascular Endothelial Cells

Blood Brain Barrier (BBB) is an important feature of the cerebral vasculature. Cells that form the barrier are called Brain Microvascular Endothelial Cells (BMECs). The barrier regulates the transport of molecular chemicals (including metabolites) and cells between the brain and blood. The barrier’s functionality is conducted via specialized groups of tight junction proteins and transporters. Found in diseases like Multiple Sclerosis, Alzheimer’s, Parkinson’s, Epilepsy, and stroke, the delicate balance between brain and blood is perturbed and BBB dysfunction can play a significant role in the disease progression.

Tempo-iBMEC™: human iPSC-derived Brain Microvascular Endothelial Cells (iBMECs) are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction condition. Tempo’s iBMEC™ cells are compact cellular structures when plated as a monolayer in culture and express standard biomarkers such as ECadherin and ZO-1 using immunohistochemistry methods and transporter biomarkers such as ABCB1 (P-gp), ABCG2, ABCE1, HIF1A, CLDN5, GLUT1, LAT1, MCT1, TJP1, PDGFbeta, OCLN, SLC25A3, TFR1, and SLC25A5 using RT/qPCR methods.