Human iPSC-derived Brain Microvascular Endothelial Cells
Blood Brain Barrier (BBB) is an important feature of the cerebral vasculature. Cells that form the barrier are called Brain Microvascular Endothelial Cells (BMECs). The barrier regulates the transport of molecular chemicals (including metabolites) and cells between the brain and blood. The barrier’s functionality is conducted via specialized groups of tight junction proteins and transporters. Found in diseases like Multiple Sclerosis, Alzheimer’s, Parkinson’s, Epilepsy, and stroke, the delicate balance between brain and blood is perturbed and BBB dysfunction can play a significant role in the disease progression.
Tempo-iBMEC™: human iPSC-derived Brain Microvascular Endothelial Cells (iBMECs) are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction condition. Tempo’s iBMEC™ cells are compact cellular structures when plated as a monolayer in culture and express standard biomarkers such as ECadherin and ZO-1 using immunohistochemistry methods and transporter biomarkers such as ABCB1 (P-gp), ABCG2, ABCE1, HIF1A, CLDN5, GLUT1, LAT1, MCT1, TJP1, PDGFbeta, OCLN, SLC25A3, TFR1, and SLC25A5 using RT/qPCR methods.
iBMEC™, anti-E-cadherin-biomarker, ICC
iBMEC™, anti-ZO1-biomarker, ICC
iBMEC™-iAstro™-iPeri™, tri-culture, transwells, TEER measurements
iBMEC™, antibody penetration, data
iBMEC™, penetration by size, kDa, measurements
iBMEC™, transwells, TEER measurements, ohm-cm2
iBMEC™, drug transporter assay 1, MK571 inhibitor
iBMEC™, drug transporter assay 2, Elacridar effects
iBMEC™-iAstro™-iPeri™, tri-culture, transwells, illustration
Tempo-iBMEC™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
in vitro Blood Brain Barrier assays
Functional Studies of CNS function
~1×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Starting Materials: human dermal/fibroblast cells were reprogrammed using a proprietary foot-print-free technology.
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
QC: Sterility, Safety; HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)