TempoCal™

Calcium Biosensor Assay

TempoCal™

Calcium Biosensor Assay

TempoCal™ is a calcium biosensor that responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm) . TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoCal™ + Tempo-iAstro™

TempoCal™ Calicum Biosensor Assay using Human Astrocytes

TempoCal™-iAstro: Tempo’s calcium biosensors are incorporated into induced human astrocytes. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm). With excellent signal-to-noise ratios, these biosensors operate on second-to-minute scales.

Tempo’s calcium biosensors are incorporated into human iPS-derived astrocytes. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission), measuring fluctuations in cytosolic calcium concentrations. “Calcium Wave” is a key feature of functional human astrocytes. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput assay platforms.

TempoCal ™ + Tempo iNStem™

TempoCal™ Calcium Biosensor Assay using Human Neural Progenitors

TempoCal™ calcium biosensors are incorporated into human neural progenitor cells. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoCal™-iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We’ve validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoCal™ biosensor is introduced to the cells using proprietary liposome mediated methods.

TempoCal™ + TempoRapid™

Tempocal™ Calcium Biosensor Assay using Immortalized Human Neuroepithelial Cells

TempoCal™ is a calcium biosensor that responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm) . It is incorporated into Tempo’s proprietary immortalized human cell line, TempoRapid™. TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

Tempo’s calcium biosensor responds in the 605nm-625nm fluorescent range (Excitation/Emission) and is incorporated into Tempo’s proprietary immortalized human cell line, TempoRapid™. TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor.

TempoCal™-control cells

TempoCal™-iAstro: stimulated with 20uM Histamine

TempoCal™-iAstro: stimulated with 20uM Histamine

TempoCal-iNStem™

TempoCal™-iAstro: quantified with stimulation

TempoCal™ and TempoMito™

TempoCal™ functions in HEK293t cells

TempoCal-iNStem™

TempoCal™-iAstro; 1mM CaCl2

TempoCal™ functions in HEK293t cells

Applications

TempoCal™ is intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.


Neurotoxicity testing for reporting cellular calcium fluctuations

Chemical compound or small molecules testing or screening

Research and Development on animal models


Phenotypic Assays


Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction pathways


Control cell line with biosensor incorporated (e.g., establishing baseline response levels, testing expression constructs)


For single-cell applications, as well as medium to high-throughput screening platforms


Studying astrocyte disorders, astrocyte differentiation and function, and calcium-mediated signal transduction pathways

Specifications

~1×10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

TempoCal™-iAstro SKU106
TempoCal™-iNStem SKU104
TempoCal™-Rapid SKU108

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