Human iPSC-derived Pericytes
In the human brain, cerebrovascular function is critical for CNS function. Pericytes belong to an important component of the neurovascular unit. Pericytes function as microcirculation cells and they are critical for the neurovascular unit (which includes brain microvascular endothelial cells, astrocytes and neurons). They communicate with endothelial cells of the small blood vessels and regulate the blood brain barrier. Dysfunctions or deficiencies in Pericytes lead to the breakdown of Blood Brain Barrier. Pericytes are integrators, coordinators and effectors of neurovascular functions including the regulation of the BBB permeability, regulation of cerebral blood flow and clearance of toxin.
Tempo-iPeri™ are human iPSC derived from nucleic-acids-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural-glial induction condition. Tempo’s iPeri™ cells are polarized compact cellular structures when plated as a monolayer in cell culture and express biomarkers such as CNPase and ZO-1 using (ICC) immunohistochemistry methods and additional biomarkers such as EDNRA, ENPEP, GRM8, KCNE4, NDUFA4L2, P2RY14, PLXDC1, RGS5, TRPC3, and PLXDC1 using RT/qPCR methods.
iPeri™, anti-ZO1-biomarker, ICC
iPeri™, anti-CNPase-biomarker, ICC
iBMEC™-iAstro™-iPeri™, tri-culture, transwells, TEER measurements
iBMEC™-iAstro™-iPeri™, tri-culture, transwells, illustration
Tempo–iPeri™ are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.
in vitro Blood Brain Barrier assays
Functional Studies of CNS function
~1×10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Starting Materials: human dermal/fibroblast cells were reprogrammed using a proprietary foot-print-free technology.
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
QC: Sterility, Safety; HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)