TempoATP™-Rapid: ATP Biosensor Assay

TempoATP™, a fluorescent biosensor, is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function. TempoATP™-Rapid cells serve as a control for all products containing the TempoATP™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.

For additional human cell lines and human cell types, please contact Tempo Support <support@tempobioscience.com>.

For filter-based instruments, Excitation/Emission: 619nm/635nm (Red) or Excitation/Emission: 517nm/535nm (Green)
For monochromator-based instruments, Excitation 590nm +/- 25nm; Emission 655nm +/- 20nm.

In addition to TempoATP™, we offer TempoMito™ biosensor assays to measure mitochondrial calcium flux. If interested, please request info on TempoMito™.

Applications:

• Cytotoxicity Assays

• Chemical compound or small molecules screening

• Cellular Metabolism Assays

• Live cell imaging (time-course studies and drug treatments).

• For low, medium, or high-throughput applications.

• Easily transfected cells to test gene-of-interest function(s).

Academic scientists receive a special rate!

Specifications: ~0.6x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility; Safety; BioSafety Level 2; HIV/viruses, bacteria, fungi = negative; Cell viability post-thawing (>90%)

Product Use:
TempoATP™-Rapid cells are intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Prefer a custom cell line product? Contact <support@tempobioscience.com>. Check out our new citations! 

SKU401

Frequently Requested Cell LInes:

Citation(s):

Bedaquiline, an FDA-approved drug, inhibits mitochondrial ATP production and metastasis in vivo, by targeting the gamma subunit (ATP5F1C) of the ATP synthase. 

Reference(s):
Martin LJ. Prog Mol Biol Transl Sci. 2012;107:355-415.
Martin LJ. Pharmaceuticals (Basel). 2010;3(4):839-915.
Chen K, Northington FJ, Martin LJ. Brain Struct Funct. 2010 Mar;214(2-3):219-34.
Palomo GM, Manfredi G. Brain Res. 2015 May 14;1607:36-46.
Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y. Toxicol Sci. 2008 Jun;103(2):335-45.
Dykens JA, Will Y. Drug Discov today. 2007 Sep; 12 (17-18): 777-85.