Tempo-iMG™: Human iPSC-derived Microglia

In stock
SKU
1001.1
$995.00

Tempo’s iMG™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming conditions. Tempo’s iMG™ microglia cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, A1F1(= IBA1), hGPR34, CXCR3 and CD11b/c. 

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Academic scientists receive a special rate! Find new cell culture solutions here.

Tempo-iMG™ microglia cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming conditions. Tempo’s iMG™ microglia cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, A1F1(= IBA1), hP2RY-12, TREM2, hGPR34, CXCR3, P2RX4, P2RX7, TRPM7, and CD11b/c. They exhibit phagocytosis activities (shown via LIVE-cell phagocytosis assay using fluorescent beads). Microglia are different from macrophages in many significant ways: synaptic stripping, secreting cytotoxic molecules, removing waste molecules from neurons, and signaling robust calcium wave responses.

Applications:

  • Disease modeling
  • Cytotoxicity evaluations
  • Phenotypic Screening (e.g., cytokine secretion, expression of differentiation markers, phagocytosis assays, synaptic denervations)
  • Preclinical validation studies
  • In vitro assays using microglial cells

Academic scientists receive a special rate! Find new cell culture solutions here.

Technology used: an in-house developed proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming technology.

Specifications: ~1.0x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU1001.1

Citation(s):

Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling

Cell Sources and Methods for Producing Organotypic in vitro Human Tissue Models

Probing Prodrug Metabolism and Reciprocal Toxicity with an Integrated and Humanized Multi-Tissue Organ-on-a-Chip Platform

Cross-talk between microglia and neurons regulates HIV latency

Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

Transport of ultrasmall gold nanoparticles (2 nm) across the blood–brain barrier in a six-cell brain spheroid model

More Information
Product Use Tempo-iMG™ are intended for basic scientific research, drug discovery, and therapeutics development use only. It is not a product for human testing or diagnostics.
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