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TempoDual™-ATPMito-Rapid: Dual Biosensor Cytotoxicity Assay

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$950.00
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Summary

TempoMito™ biosensor is an mitochondrial calcium flux sensor. The cells have been engineered to express TempoMito™ biosensor which has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Excitation=610nm; Emission=625nm (Filter-based fluorescent multiplate readers). TempoMito™ biosensor is introduced to the cells using proprietary magneto-liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoATP™, a fluorescent biosensor, is also custom-incorporated into the human TempoRapid™cell line . With TempoATP™ , the biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function. Excitation=535nm; Emission=550nm (Filter-based fluorescent multiplate readers).

TempoRapid™ cells are immortalized human neural-epithelial cells. The biosensors are transiently expressed. Please send inquiries for stable cell line development to Tempo Support.

TempoATP™ and TempoMito™

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  • TempoDual™, illustration
  • TempoATP™
  • TempoMito™
  • TempoMito™-TempoATP™; TempoRapid™cells.

Details

TempoMito™ biosensor is an mitochondrial calcium flux sensor. The cells have been engineered to express TempoMito™ biosensor which has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Excitation=610nm; Emission=625nm (Filter-based fluorescent multiplate readers). TempoMito™ biosensor is introduced to the cells using proprietary magneto-liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoATP™, a fluorescent biosensor, is also custom-incorporated into the human TempoRapid™ cell line . With TempoATP™ , the biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function. Excitation=535nm; Emission=550nm (Filter-based fluorescent multiplate readers).

TempoRapid™ cells are immortalized human neural-epithelial cells. The biosensors are transiently expressed with the biosensors. Please send inquiries for stable cell line development to Tempo Support. Alternative cell lines are available. Please see Frequently Requested Cell Lines below.

Applications:

  • Cytotoxicity Assays
  • Chemical compound or small molecules screening
  • Cellular Metabolism Assays
  • Mitochondrial  metabolism research
  • Live cell imaging (time-course studies and drug treatments).
  • Cellular toxicity testing for reporting mitochondrial calcium fluctuations
  • Phenotypic Assays (i.e., mitochondrial toxicity and function)
  • Chemical compound or small molecules testing
  • Cell morphology or cell death research studies.

Academic scientists receive a special rate!

Specifications: ~0.5x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Starting Materials: immortalized human cell line. Cells grow fairly well (doubling time ~72hrs) without passage limitation in a conventional medium: DMEM with a maximum of 10% serum, 1x Essential amino acids, Pen/Strep and selection antibiotics (such as G418, hygromycin or puromycin) at experimentally determined concentrations for each user’s experiment if a gene-of-interest is introduced with a selection marker. Culture Medium: DMEM with 2mM L-Glutamine; 10% Fetal Bovine Serum (FBS). Subculture Routine: Split sub-confluent using 0.025% trypsin/EDTA solution. Allow the flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. Passage ratio: 1-to-6.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC:
Sterility, Safety (BioSafety Level 2)
HIV/viruses, bacteria, fungi: negative
Cell viability post-thawing (>90%)

SKU403

Frequently Requested Cell LInes:

Cell Line Name Histology Type
U2OS Bone carcinoma
HEK293 Immortalized Kidney Epithelial
SW 480 Colorectal adenocarcinoma
U87MG Brain cancer
Hep G2 Hepatocellular carcinoma
NIH3T3 Embroyonic fibroblast, murine
HT1080 Carcinoma (connective tissue)
HCT116 Colorectal cancer
MCF 7 Breast cancer
SW 1990 Pancreatic cancer
NCI-H2126 Non-Small cell cancer
HUVEC Umbilical vascular endothelium
HeLa Cervical epithelial adenocarcinoma
CG4 Glial cell line (from rat)
A-204 Muscle epithelial
A549 Lung epithelial cell line
BT-142 Oligoastrocytoma, suspension
NK-92 Peripheral blood, natural killer cells
CHO Chinese hamster ovarian cell line
NCI-H69 Lung tumor cell aggregate, suspension
ARPE-19 Retinal pigmented epithelial
Beta-TC-6 Beta cell (mouse)
BxPC-3 Pancreatic epithelial, adenocarcinoma
Jurkat cells Peripheral T lymphocyte, suspension
SW 780 Urinary bladder epithelial carcinoma
MeT-5A Mesothelium, from non-cancerous
BT142 Oligoastrocytoma
NM2C5 Melanocyte from melanoma
IFNgamma-TempoRapidTM ask Tempo Support
Primary B or T lymphocytes ask Tempo Support
NFKB reporters ask Tempo Support
Mesenchymal stromal cells ask Tempo Support
Chemokine Reporter cells ask Tempo Support
request cell line of your choice ask Tempo Support

 

Additional Information

Product Use TempoATP™-TempoMito™ incorporated cells are intended for research and development, compound discovery and therapeutics development use only. It is not a product for human testing or diagnostics.