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  1. popular Tempo-iCort; ICC anti-Map2 (CST)

    Tempo-iCort™: human iPS-derived cortical neurons

    $775.00

    Tempo’s iCort™: human cortical neurons are derived from human dermal cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iCort™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta-3-Tuj, GAD65/67 and Map2. iCort™ can be used under maturation conditions, co-culture conditions (with astrocytes), and short-term functional characterization studies. 

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  2. popular TempoO2-Rapid, ActinomycinA treated

    TempoO2™-Rapid: Oxygen Metabolism Biosensor Assay

    $595.00

    TempoO2™, a fluorescent biosensor, is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution: seconds to hours) in measuring real-time cellular metabolism and cytotoxicity. Analysis of cellular respiration directly reflects mitochondrial health and function. TempoO2™-Rapid cells serve as a control for all products containing the TempoO2™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest. For additional human cell lines and human cell types, please contact Tempo Support <support@tempobioscience.com>.

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  3. popular TempoATP cells, Rotenone treated

    TempoATP™-Rapid: ATP Biosensor Assay

    $595.00

    TempoATP™, a fluorescent biosensor, is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapidTM . The biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function.

    TempoATP™-Rapid cells serve as a control for all products containing the TempoATP™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.

    For additional human cell lines and human cell types, please contact Tempo Support <support@tempobioscience.com>.

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  4. popular iDopaNer, ICC data with anti-TH

    Tempo-iDopaNer™: Human iPS-derived Dopaminergic Neurons

    $850.00

    Tempo’s iDopaNer™: human dopaminergic neurons are derived from human dermal cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iDopaNer™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta3-Tuj (neuronal), tyrosine hydroxylase (TH), and dopamine transporter (DAT).  iDopaNer™ can be used under maturation conditions, co-culture conditions (with astrocytes or additional neuronal subtypes), and short-term functional characterization studies.

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  5. popular iPS-derived cortical neurons; ICC using anti-beta-Tuj

    TempoVol™-iCort: Cationic Voltage Biosensor Assay using Human Cortical Neurons

    $950.00

    TempoVol™-iCort: cationic voltage biosensor assay using human iPS-derived . They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction and maturation conditions. TempoVol™-iCort cells are polarized structures when plated as a monolayer in culture and express markers such as MAP2 and beta-Tuj. We've validated iCort™ cells to be cationic-conducting neurons when exposed to solutions with fluctuating concentrations of sodium, calcium, potassium, magnesium, and protons/H+. TempoVol™ biosensor is introduced to the cells using proprietary methods and is expressed in the in abundance in the cell. Learn More
  6. HEK293t cells incorporated with TempoMito biosensor

    TempoMito™-HEK293t

    $395.00

    HEK293t cell line is one of the most widely used human immortalized cell lines in research and drug development. The cells are very easy to work with and can perform a variety of functional applications (e.g., protein purification, expression studies, differential screening, functional reporter assays, receptor-ligand assays, etc). TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). HEK293t stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Minimum purchase requirement: 5 vials.

    Prefer a custom product instead? Choose a cell line and contact <support@tempobioscience.com>.

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  7. popular TempoRapid Cells with TempoMito

    TempoMito™-U2OS

    $395.00

    U2-OS cell line, originally known as the 2T cell line, is an immortalized human cancer cell line. It is widely used in research and drug development, as it offers a variety of application potentials. TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). U2-OS stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Minimum purchase requirement: 5 vials.

    Prefer a custom product instead? Choose a cell line and contact <support@tempobioscience.com>.

     

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  8. TempoVol responses

    TempoVol™-iNStem: Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells

    $950.00

    TempoVolTM cationic voltage biosensors are incorporated into human neural progenitor cells. They respond to intracellular ionic changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium/potassium/proton/sodium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

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  9. TempoVol in TempoRapid cells, image1

    TempoVol™-Rapid: Cationic Voltage Biosensor Assay using Immortalized Human Neuroepithelial Cells

    $595.00

    TempoVol™ cationic voltage biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission: 610nm/625nm). It is incorporated into Tempo's proprietary immortalized human neuroepithelial cell line, TempoRapidTM. TempoVol™ control cells serve as a control for all products containing the TempoVol™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

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  10. TempoMito™ -control cells image1

    TempoMito™-Rapid: Mitochondrial Biosensor Assay using Immortalized Human Neuroepithelial Cells

    $595.00

    TempoMito™ mitochondrial calcium biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range. It is incorporated into Tempo's proprietary immortalized human neuroepithelial cell line, TempoRapidTM. TempoMito™ control cells serve as a control for all products containing the TempoMito™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

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