TempoVol™-iNStem: Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells

In stock

TempoVolTM cationic voltage biosensors are incorporated into human neural progenitor cells. They respond to intracellular ionic changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium/potassium/proton/sodium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission: 610nm/625nm) and is incorporated into Tempo's iPSC-derived neural progenitor cells, iNStem. iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoVol™ biosensor is introduced to the cells using proprietary liposome mediated methods.


  • Chemical compound or small molecules testing
  • Phenotypic Assays
  • Neurotoxicity testing for reporting cationic flux
  • Signal transduction pathway modulations
  • Ion Channel studies
  • Chemical compound or small molecules testing or screening
  • For low, medium, or high-throughput applications

Specifications: ~2x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

QC: Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.


More Information
Product Use TempoVol™-iNStem are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.