TempoVol™-Rapid: Cationic Voltage Biosensor Assay using Immortalized Human Neuroepithelial Cells
TempoVol™ cationic voltage biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission: 610nm/625nm). It is incorporated into Tempo's proprietary immortalized human neuroepithelial cell line, TempoRapidTM. TempoVol™ control cells serve as a control for all products containing the TempoVol™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.
TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission: 610nm/625nm) and is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapidTM. TempoVol™-Rapid cells serve as a control for all products containing the TempoVol™ biosensor.
- Chemical compound or small molecules testing
- Phenotypic Assays
- Control cell line with biosensor incorporated (e.g., establishing baseline response levels, testing expression constructs)
- For low, medium, or high-throughput applications
Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Starting Materials: immortalized human cell line of neuro-epithelial origin. Cells should grow fairly well (doubling time < 36 h) without passage limitation in a conventional medium: DMEM or DMEM/F12 media with a maximum of 10% serum, 1x essential amino acids, Pen/Strep and selection antibiotics (such as G418, hygromycin or puromycin) at experimentally determined concentrations for each user’s experiment if a gene-of-interest is introduced with a selection marker.
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
|Product Use||TempoVol™-control cells are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.|