TempoMito™-iAstro: Mitochondrial Biosensor Assay using Human Astrocytes
TempoMito™-iAstro cells contain mitochondrial biosensors in human iPS-derived astrocytes. Disruption of mitochondrial functions in human astrocytes has been implicated in Alzheimer's disorder and ischemia. Tempo's biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening.
TempoMito™-iAstro: Mitochondrial Calcium Biosensor Assay using human Astrocytes
TempoMito™-iAstro are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary astrocytes induction condition. TempoMito™-iAstro cells are plated as monolayers in culture and express markers such as GFAP. TempoMito™ biosensor is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).
- Neurotoxicity testing for reporting mitochondrial calcium fluctuations
- Phenotypic Screening (e.g., mitochondrial toxicity)
- Chemical compound or small molecules testing
- Research and Drug Development for human mitochondrial diseases, myelin disorders and neurodegnerative disorders
- Studying astrocyte differentiation and function, and calcium-mediated signal transduction pathways
Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.
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|Product Use||TempoMito™-iAstro are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.|