TempoMito™-HEK293t
HEK293t cell line is one of the most widely used human immortalized cell lines in research and drug development. The cells are very easy to work with and can perform a variety of functional applications (e.g., protein purification, expression studies, differential screening, functional reporter assays, receptor-ligand assays, etc). TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). HEK293t stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter.
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HEK293t cell line is one of the most widely used human immortalized cell lines in research and drug development. The cells are very easy to work with and can perform a variety of functional applications (e.g., protein purification, expression studies, differential screening, functional reporter assays, receptor-ligand assays, etc).
TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). HEK293t stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter.
Culture Medium: DMEM with 2mM L-Glutamine; 10% Fetal Bovine Serum (FBS). Subculture Routine: Split sub-confluent using 0.025% trypsin/EDTA solution. Allow the flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. Passage ratio: 1-to-9.
Applications:
- Cellular toxicity testing for reporting mitochondrial calcium fluctuations
- Phenotypic Assays (i.e., mitochondrial toxicity and function)
- Chemical compound or small molecules testing
- Cell morphology or cell death research studies.
- High Content Assays
- Live Cell Imaging
- Mitochondrial metabolism
- Cellular metabolism
QC: Sterility, Safety, HIV/viruses, bacteria, fungi: (negative), Cell viability post-thawing (>90%).
1 vial = ~0.5x10^6 cells
SKU302
References:
Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y. Toxicol Sci. 2008 Jun;103(2):335-45.
Dykens JA, Will Y. Drug Discov today. 2007 Sep; 12 (17-18): 777-85.
Prefer a custom cell line product? Contact <support@tempobioscience.com>
Cell Line Name | Histology Type | |
U2OS | Bone carcinoma | |
HEK293 | Immortalized Kidney Epithelial | |
SW 480 | Colorectal adenocarcinoma | |
U87MG | Brain cancer | |
Hep G2 | Hepatocellular carcinoma | |
NIH3T3 | Embroyonic fibroblast, murine | |
HT1080 | Carcinoma (connective tissue) | |
HCT116 | Colorectal cancer | |
MCF 7 | Breast cancer | |
SW 1990 | Pancreatic cancer | |
NCI-H2126 | Non-Small cell cancer | |
HUVEC | Umbilical vascular endothelium | |
HeLa | Cervical epithelial adenocarcinoma | |
CG4 | Glial cell line (from rat) | |
A-204 | Muscle epithelial | |
A549 | Lung epithelial cell line | |
BT-142 | Oligoastrocytoma, suspension | |
NK-92 | Peripheral blood, natural killer cells | |
CHO | Chinese hamster ovarian cell line | |
NCI-H69 | Lung tumor cell aggregate, suspension | |
ARPE-19 | Retinal pigmented epithelial | |
Beta-TC-6 | Beta cell (mouse) | |
BxPC-3 | Pancreatic epithelial, adenocarcinoma | |
Jurkat cells | Peripheral T lymphocyte, suspension | |
SW 780 | Urinary bladder epithelial carcinoma | |
MeT-5A | Mesothelium, from non-cancerous | |
Mesenchymal stromal cells | email <support@tempobioscience.com> | |
Chemokine Reporter cells | email <support@tempobioscience.com> | |
cell line of your choice | email <support@tempobioscience.com> |
Product Use | TempoMito™-HEK293 are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics. |
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