TempoMito™-HEK293t

HEK293t cell line is one of the most widely used human immortalized cell lines in research and drug development. The cells are very easy to work with and can perform a variety of functional applications (e.g., protein purification, expression studies, differential screening, functional reporter assays, receptor-ligand assays, etc).

TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). HEK293t stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter.

Culture Medium: DMEM with 2mM L-Glutamine; 10% Fetal Bovine Serum (FBS). Subculture Routine: Split sub-confluent using 0.025% trypsin/EDTA solution. Allow the flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. Passage ratio: 1-to-9.

Applications:

• Cellular toxicity testing for reporting mitochondrial calcium fluctuations
• Phenotypic Assays (i.e., mitochondrial toxicity and function)
• Chemical compound or small molecules testing
• Cell morphology or cell death research studies.
• High Content Assays
• Live Cell Imaging
• Mitochondrial metabolism 
• Cellular metabolism 

QC: Sterility, Safety, HIV/viruses, bacteria, fungi: (negative), Cell viability post-thawing (>90%).

1 vial = ~0.5x10^6 cells

SKU302

References:

Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y. Toxicol Sci. 2008 Jun;103(2):335-45. 

Dykens JA, Will Y. Drug Discov today. 2007 Sep; 12 (17-18): 777-85.

Prefer a custom cell line product? Contact <support@tempobioscience.com>