TempoCal™-iNStem: Calcium Biosensor Assay using Human Neural Progenitor Cells
TempoCalTM calcium biosensors are incorporated into human neural progenitor cells. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.
TempoCal™-iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoCal™ biosensor is introduced to the cells using proprietary liposome mediated methods.
- Neurotoxicity testing for reporting cellular calcium fluctuations
- Chemical compound or small molecules testing or screening
- Research and Development on animal models
- Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction pathways.
Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
|Product Use||TempoCal™-iNStem are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.|