Tempo-iAstro™: Human iPSC-derived Astrocytes

In stock
SKU
101
$895.00

Astrocytes, also known as Astroglia, belong to the family of glial cells in the human central and peripheral nervous system. They have been shown to have central roles in the maintenance of neuronal metabolism and neurotransmitter synthesis. They have been implicated in many human neurodegenerative disorders such as multiple sclerosis, amyotrophic lateral sclerosis (ALS), Alzheimer’s disorder, Huntington’s disease and Parkinson’s disease.

Tempo’s iAstro™: human iPSC-derived astrocytes are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iAstro cells are polarized structures when plated as a monolayer in culture and express astrocyte markers, GFAP, EAAT1, Aquaporin family genes, and S100beta, and they exhibit calcium wave activities (see figures shown above). There are two subtypes of human astrocytes: Type I and Type II. >90% of Tempo-iAstro™ astrocytes are Type II. If you need Type I astrocytes, please email Tempo Support.

Check out our new citations! 

Academic scientists receive a special rate! Find new cell culture solutions here.

Astrocytes, also known as Astroglia, belong to the family of glial cells in the human central and peripheral nervous system. They have been shown to have central roles in the maintenance of neuronal metabolism and neurotransmitter synthesis. They have been implicated in many human neurodegenerative disorders such as multiple sclerosis, amyotrophic lateral sclerosis (ALS), Alzheimer’s disorder, Huntington’s disease and Parkinson’s disease.

Tempo’s iAstro™: human iPSC-derived astrocytes are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iAstro cells are polarized structures when plated as a monolayer in culture and express astrocyte markers, GFAP, EAAT1, Aquaporin family genes, and S100beta, and they exhibit calcium wave activities (see figures shown above). There are two subtypes of human astrocytes: Type I and Type II. >90% of Tempo-iAstro™ astrocytes are Type II. If you need Type I astrocytes or neuron-astrocyte cocultures, please email Tempo Support.

Applications:

  • Disease modeling 
  • Toxicity testing
  • Phenotypic Screening (e.g., cytokine secretion, expression of differentiation markers)
  • Research and development of cell therapies in animal models
  • Studying astrocyte differentiation and function

Academic scientists receive a special rate! Find new cell culture solutions here.

Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary non-viral, nucleic-acids-free, feeder-free, serum-free, and integration-free reprogramming technology.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU101

Citations:

1) Enhanced Astrocyte Responses are Driven by a Genetic Risk Allele Associated with Multiple Sclerosis

2) The Genetic Multiple Sclerosis (MS) Risk Variant rs7665090-G Increases Astrocyte Responses and Lymphocyte Recruitment to White Matter Lesions.

3) The Role of Astrocytes in Multiple Sclerosis. (Review)

4) New Research to Uncover Cellular Mechanisms Driving Risk for Multiple Sclerosis. (Review)

5) Cell Sources and Methods for Producing Organotypic in vitro Human Tissue Models

6) Patient-Derived Orthotopic Xenografts and Cell Lines from Pediatric High-Grade Glioma Recapitulate the Heterogeneity of Histopathology, Molecular Signatures, and Drug Response. 

7) βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

More Information
Product Use Tempo’s iAstro™ cells are intended for scientific research and drug development use only. It is not a product for human therapeutics or diagnostics.