Custom Engineered Cell Models

Tempo-iCardio™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines using a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.  They are cultured as monolayers and are alive for >4 weeks under defined cell culture conditions. Tempo-iCardio™ cells express biomarkers: ACTC1, CSPR3, KCNQ1, MYBPC3, MYH7, MYL2, PKP2, PRKAG2, TNNI3, TNNT, TPMI, TTN. Immunocytochemistry data show the expressions of TNNT2 and E-Cadherin.

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If you would like to purchase them, please contact us to discuss your particular requirements. Please inquire for ready-to-ship Human iPSC-derived Hepatocytes. 96-well format. Ethnically diverse lines available.

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Tempo-iKidneyPod™ introduces a complex biological culture system in a 3D spheroid model system which improves the physiological relevance of in vitro renal proximal tubule epithelium models. Tempo-iKidneyPod™ are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free method. They are 3D spheroid structures and are alive for >4 weeks under defined cell culture conditions. They express biomarkers:  CLDN1, NPHS1, OAT1, OAT3, OCT2, PAX8, Podocin, and Synaptopodin. Additional biomarkers expressed include: KCNJ1, LHX1, MCCD1, OSR1, Pax2, PodXL, Six2, FXYD4, MIOX, Pax8, SLC6A18, SLC12A1, SLC34A1, TMEM207, TMEM174, and UMOD (mRNA data not shown). Transporter functions of the Tempo-iKidneyPod™ are evaluated using transporter dyes: 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (4-Di-1-ASP) to measure OCT2 uptake, and 6-carboxy-fluorescein (6-CF) to measure OAT1/3 uptake. TempoChloro™ biosensor is used to measure chloride flux.

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Tempo-iOsteo™ are reprogrammed using our pproprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology. They expression biomarkers: Osteocalcin, COL1A1, FLT1, Bone Sialoprotein, RUNX2, and SPP1 (q/RT-PCR data not shown). Bone mineralization data (Alizarin S Red; 530nm absorbance data) are shown.

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Please inquire for ready-to-ship Tempo-iOsteo™ lines. Regarding patients derived iOsteo cells, browse our TempoStemBank™-iPSC collection. 

Customized Reporter Cell Lines for TempoCal, TempoVol, TempoMito, TempoATP, or TempoO_2. Pairing Instructions: Step 1: Decide on a cell line. Step 2: Choose a Tempo Biosensor: TempoCal, TempoVol, TempoMito, TempoATP, or TempoO₂ Step 3: Need multiple biosensors per cell line? Step 4: Decide on the number of cells needed. Step 5: Email Tempo Support <support@tempobioscience.com>. Congratulations! You are done. Let us help you move your project forward! (Need a complete kit? Just ask us!)

TempoStemBank™-iPSC: human iPSC lines are derived from human dermal/fibroblasts, blood or cord blood, using our proprietary genetic/viral-elements-free, nucleic-acids-free, feeder-free, and serum-free reprogramming technology. The cells express canonical biomarkers of iPSCs (e.g., Oct4, Tra-1-60, Tra-1-81, Sox2, Nanog, and SSEA-4); they have been proven to be multipotent and are able to differentiate into ectoderm, endoderm and mesoderm derivative cell types. Please inquire for ready-to-ship low passage iPSCs (Free cell culture media included!). 

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