Tempo-iMSC™: Human iPSC-derived Mesenchymal Stem Cells

Mesenchymal stem cells ("MSCs") are non-haematopoietic, multipotent stromal stem cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, myocytes, and adipocytes. Tempo-iMSC^TM are human iPSC-derived mesenchymal stem cells. Tempo-iMSC^TM express cell surface markers such as CD73, CD90, CD166, and CD105, in addition to Oct4 and Nanog. This expression profile is predictive of the long-term proliferative capacity of cultured human MSCs (see reference: here). MSCs have immunomodulatory features, secrete cytokines and immune-receptors which regulate the microenvironment in the host tissue and they have multi-lineage potentials for a variety of cell types. MSCs can be used in a variety of screening applications, from screening biologics and small molecules to siRNAs. Due to MSCs' ability to self-renew (= growth without differentiation), they are easily scalable for running large-scale assays.

NEW! For scientists working on exosomes, please email us for conditional media for exosome extractions and biomarker validations.

NEW! For scientists working on CD34+ HSCs, please email us regarding Tempo-iHSC^TM (SKU214; Custom Requests Only).

Applications:

• Developmental studies
• Biomarker discoveries and validations
• Lineage differentiation studies
• Large-scale compound screenings
• In vitro assays using MSCs (e.g., cytokine release assays)

Academic scientists receive a special rate! Find new cell culture solutions here.

Specifications: ~1.25 x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent monolayer

Technology Used: in-house developed proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.

Starting Materials: human dermal/fibroblast cells

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety, HIV/viruses, bacteria, fungi: negative, cell viability post-thawing (>85%).

SKU209