Human Cell Models

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  1. Tempo-iMotorNers, anti-acetylcholinesterase antibody against human AchT protein
    Tempo-iMotorNer™: Human iPSC-derived Motor Neurons
    $925.00

    Tempo’s iMotorNer™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology conditions. Motor neurons have synapses that transmit via cholinergic transmitters. These synapses require extremely fast inactivations of its transmitter, acetylcholine (Ach), by utilizing the enzyme, acetylcholinesterase (AchT). AchT hydrolyzes acetylcholine to acetate and choline, as a way of inactivation. Tempo’s iMotorNer™ cells are polarized structures when plated as a monolayer in culture and express biomarkers, acetylcholinesterase (AchT), beta-TUJ (neuron-specific), Isl1 and HB9.

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  2. Tempo-iMG, ICC, anti-human-CXCR3 antibody
    Tempo-iMG™: Human iPSC-derived Microglia
    $995.00

    Tempo’s iMG™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming conditions. Tempo’s iMG™ microglia cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, A1F1(= IBA1), hGPR34, CXCR3 and CD11b/c. 

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  3. Tempo-iKer, ICC using anti-human-CK18, image 3
    Tempo-iKer™: Human iPSC-derived Keratinocytes
    $805.00

    Tempo-iKer™: human keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, genetic-elements-free, virus-free,  induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iKer™ cells are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratin-18. iKer™ can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.

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  4. iMel cells, ICC using anti-human-Vimentin
    Tempo-iMel™: Human iPSC-derived Melanocytes
    $825.00

    Tempo-iMel™: human melanocytes are derived from human induced pluripotent stem cell (iPSC) lines under a fully defined proprietary reprogramming and maturation conditions. Tempo’s iMel™ cells are branched elaborate structures when plated as a monolayer in culture and express biomarkers such as Vimentin, S100beta, and cytokeratins. iMel™ can be used under maturation conditions, co-culture conditions (with fibroblasts, keratinocytes or additional epidermal subtypes), and short-term functional characterization studies. 

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  5. Human iPSC Lines, anti-human-Oct4, ICC
    TempoStemBank™-iPSC: Human iPSC Stem Cell Lines

    TempoStemBank™-iPSC: human iPSC lines are derived from human dermal/fibroblasts, blood or cord blood, using our proprietary genetic/viral-elements-free, nucleic-acids-free, feeder-free, and serum-free reprogramming technology. The cells express canonical biomarkers of iPSCs (e.g., Oct4, Tra-1-60, Tra-1-81, Sox2, Nanog, and SSEA-4); they have been proven to be multipotent and are able to differentiate into ectoderm, endoderm and mesoderm derivative cell types. Please inquire for ready-to-ship low passage iPSCs (Free culture media included!). 

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  6. Custom Hepatocytes; anti-E-Cadherin antibody
    Tempo-iHep™: Human iPSC-derived Hepatocytes

    If you would like to purchase them, please contact us to discuss your particular requirements. Please inquire for ready-to-ship Human iPSC-derived Hepatocytes. 96-well format. Ethnically diverse lines available.

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  7. iDopaNer, ICC data with anti-TH
    Tempo-iDopaNer™: Human iPSC-derived Dopaminergic Neurons
    $875.00

    Tempo’s iDopaNer™: human dopaminergic neurons are derived from human dermal cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iDopaNer™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta3-Tuj (neuronal), tyrosine hydroxylase (TH), and dopamine transporter (DAT).  iDopaNer™ can be used under maturation conditions, co-culture conditions (with astrocytes or additional neuronal subtypes), and short-term functional characterization studies. 

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  8. Tempo-iCort; ICC anti-Map2 (CST)
    Tempo-iCort™: Human iPSC-derived Cortical Neurons
    $885.00

    Tempo’s iCort™: human cortical neurons are derived from human dermal cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iCort™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta-3-Tuj, GAD65/67, VGLUT1/2, and Map2. iCort™ can be used under maturation conditions, co-culture conditions (with astrocytes), and short-term functional characterization studies. 

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  9. iOligo (O4 marker, Green)
    Tempo-iOligo™: Human iPSC-derived Oligodendrocyte Progenitor Cells (OPCs)
    $940.00

    Oligodendrocytes belong to the family of glial cells in the human central and peripheral nervous system. They are multifunctional cells and are implicated in many disabling neurological disorders, including adult-onset demyelinating disorders, as well as leukodystrophies and cerebral palsy.

    Tempo’s iOligo™ are induced oligodendrocyte progenitors reprogrammed from human fibroblasts cells using a proprietary reprogramming technology. iOligo™ cells are cryopreserved at low passage numbers. Each vial contains ~1.05 x 10cells. iOligo™ cells are characterized via standard morphological examinations and via immunocytochemistry methods against known marker proteins (such as O4, PDGFalphaR, NG2, and CNPase) and can be induced to express MBP upon T3 hormone induction.

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  10. iAstro (TM) human astrocytes, light transmission
    Tempo-iAstro™: Human iPSC-derived Astrocytes
    $829.00

    Astrocytes, also known as Astroglia, belong to the family of glial cells in the human central and peripheral nervous system. They have been shown to have central roles in the maintenance of neuronal metabolism and neurotransmitter synthesis. They have been implicated in many human neurodegenerative disorders such as multiple sclerosis, amyotrophic lateral sclerosis (ALS), Alzheimer’s disorder, Huntington’s disease and Parkinson’s disease.

    Tempo’s iAstro™: human iPSC-derived astrocytes are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iAstro cells are polarized structures when plated as a monolayer in culture and express astrocyte markers, GFAP, EAAT1, Aquaporin family genes, and S100beta, and they exhibit calcium wave activities (see figures shown above). There are two subtypes of human astrocytes: Type I and Type II. >90% of Tempo-iAstro™ astrocytes are Type II. If you need Type I astrocytes, please email Tempo Support.

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