Human Cell Models

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  1. Cell Culture Solutions
    Cell Culture Solutions

    Our qualified cell culture media are "ready-to-use" formulations, specifically designed and optimized for your chosen cell types. Simply select your cell type and email us for cell culture solutions!

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  2. Tempo-iSenso and Tempo-iSchwann co-culture, anti-MBP biomarker (ICC)
    Tempo-iSenso™: Human iPSC-derived Sensory Neurons
    $985.00

    Tempo-iSenso™ cells are human sensory neurons derived from human dermal cells, using human induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iSenso™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta-Tuj (neuronal specific), BRN3a and Peripherin. In addition, Tempo-iSenso™ cells express biomarkers such as ASIC, CNR1, NTRK, P2X4, SCN9a/Nav1.7, TRPA1, TRPV1, P2X3, and SCN10A/Nav1.8 (RT/qPCR data not shown).  iSenso™ can be used under maturation conditions or co-culture conditions (with Schwann cells or additional neuronal subtypes) for a variety of functional characterizations and drug candidate validation studies.  

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  3. Tempo-iSchwann™ anti-S100b antibody
    Tempo-iSchwann™: Human iPSC-derived Schwann Cell
    $905.00

    Tempo-iSchwann™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, genetic-vector-free, and integration-free conditions. They are maintained as a monolayer in cultures under defined cell culture conditions and they can be co-cultured with neurons. Tempo-iSchwann™ express biomarkers: GAP43, MPZ, NCAM1, NGFR, OCT6, Sox2, and Sox10 (mRNA analysis). Shown using immunocytochemistry, Tempo-iSchwann™ express S100 beta and MBP. 

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  4. Tempo-iPhago™ LIVE-Cell phagocytosis
    Tempo-iPhago™: Human iPSC-derived Phagocytes
    $925.00

    Tempo-iPhago™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, genetic-vector--free, and integration-free conditions. They can be maintained as monolayer or suspension cultures under defined cell culture conditions. Tempo-iPhago™ express CD14, CD16, CD64, CD68, CD71, CD115, CD156c, and CD204. Tempo-iPhago™ show phagocytosis activities via LIVE-cell FITC-labelled beads uptake.

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  5. Tempo-iMono™ anti-CD11b antibody
    Tempo-iMono™: Human iPSC-derived CD14+ Monocytes
    $905.00

    Tempo-iMono™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines using a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.  They are maintained as suspension cultures under defined cell culture conditions. Tempo-iMono™ cells express CD14, CD68, CD71, CD115, and CD156c. Tempo-iMono™ also express CD11b and CXCR3.

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  6. Tempo-iCardio™ anti-TNNT2 antibody (ThermoFisher)
    Tempo-iCardio™: Human iPSC-derived Cardiomyocytes

    Tempo-iCardio™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines using a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.  They are cultured as monolayers and are alive for >4 weeks under defined cell culture conditions. Tempo-iCardio™ cells express biomarkers: ACTC1, CSPR3, KCNQ1, MYBPC3, MYH7, MYL2, PKP2, PRKAG2, TNNI3, TNNT, TPMI, TTN. Immunocytochemistry data show the expressions of TNNT2 and E-Cadherin.

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  7. Tempo-iKidneyPod™ with 6-CF dye (OAT1/3 uptake)
    Tempo-iKidneyPod™: Human iPSC-derived Kidney Proximal Tubules and Podocyte 3D Spheroids

    Tempo-iKidneyPod™ introduces a complex biological culture system in a 3D spheroid model system which improves the physiological relevance of in vitro renal proximal tubule epithelium models. Tempo-iKidneyPod™ are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free method. They are 3D spheroid structures and are alive for >4 weeks under defined cell culture conditions. They express biomarkers:  CLDN1, NPHS1, OAT1, OAT3, OCT2, PAX8, Podocin, and Synaptopodin. Additional biomarkers expressed include: KCNJ1, LHX1, MCCD1, OSR1, Pax2, PodXL, Six2, FXYD4, MIOX, Pax8, SLC6A18, SLC12A1, SLC34A1, TMEM207, TMEM174, and UMOD (mRNA data not shown). Transporter functions of the Tempo-iKidneyPod™ are evaluated using transporter dyes: 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (4-Di-1-ASP) to measure OCT2 uptake, and 6-carboxy-fluorescein (6-CF) to measure OAT1/3 uptake. TempoChloro™ biosensor is used to measure chloride flux.

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  8. Tempo-iOsteo, healthy donor, Alizarin S Red assay
    Tempo-iOsteo™: Human iPSC-derived Osteoblasts

    Tempo-iOsteo™ are reprogrammed using our pproprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology. They expression biomarkers: Osteocalcin, COL1A1, FLT1, Bone Sialoprotein, RUNX2, and SPP1 (q/RT-PCR data not shown). Bone mineralization data (Alizarin S Red; 530nm absorbance data) are shown.

    Academic scientists receive a special rate! Find new cell culture solutions here.

    Please inquire for ready-to-ship Tempo-iOsteo™ linesRegarding patients derived iOsteo cells, browse our TempoStemBank™-iPSC collection. 

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  9. Tempo-iMSC, monolayer, 9 days post-thaw
    Tempo-iMSC™: Human iPSC-derived Mesenchymal Stem Cells
    $895.00

    Mesenchymal stem cells ("MSCs") are non-haematopoietic, multipotent multipotent stromal stem cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, myocytes, and adipocytes. MSCs express cell surface markers such as CD73, CD90, CD166, and CD105, in addition to Oct4 and Nanog. Tempo-iMSC™ are human iPSC-derived mesenchymal stem cells. NEW! For scientists working on exosomes, please contact us for conditional media for exosome extractions and biomarker validations.

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  10. Tempo-iRPE; immunocytochemistry, anti-human-RPE65 (ThermoFisher)
    Tempo-iRPE™: Human iPSC-derived Retinal Pigment Epithelials
    $825.00

    Retinal pigment epithelium (RPE) is the pigmented cell layer surrounding the neurosensory retina. The cells support the retinal visual cells, and they are attached to the underlying choroid and overlying retinal visual cells. The RPE has several functions: light absorption, epithelial transport, spatial ion buffering, visual cycle, phagocytosis, secretion and immune modulation. Tempo-iRPE™ are human iPSC-derived retinal pigment epithelial cells. They express biomarkers such as RPE-65, RLBP1, and BEST.

    Academic scientists receive a special rate! Find new cell culture solutions here.

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