TempoVol™-iNStem: Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells
TempoVolTM cationic voltage biosensors are incorporated into human neural progenitor cells. They respond to intracellular ionic changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium/potassium/proton/sodium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.
TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission: 610nm/625nm) and is incorporated into Tempo's iPSC-derived neural progenitor cells, iNStem. iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoVol™ biosensor is introduced to the cells using proprietary liposome mediated methods.
Applications:
- Chemical compound or small molecules testing
- Phenotypic Assays
- Neurotoxicity testing for reporting cationic flux
- Signal transduction pathway modulations
- Ion Channel studies
- Chemical compound or small molecules testing or screening
- For low, medium, or high-throughput applications
Specifications: ~2x10^6 cells per 1ml of freezing medium (vial)
Long-term Storage: liquid nitrogen
Growth Properties: adherent
Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.
Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.
SKU121
| Product Use | TempoVol™-iNStem are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures. |
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