Biosensors

TempoMito™ biosensor is an mitochondrial calcium flux sensor. The cells have been engineered to express TempoMito™ biosensor which has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Excitation=610nm; Emission=625nm (Filter-based fluorescent multiplate readers). TempoMito™ biosensor is introduced to the cells using proprietary magneto-liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoATP™, a fluorescent biosensor, is also custom-incorporated into the human TempoRapid™cell line . With TempoATP™ , the biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function. Excitation=535nm; Emission=550nm (Filter-based fluorescent multiplate readers).

TempoRapid™ cells are immortalized human neural-epithelial cells. The biosensors are transiently expressed. Please send inquiries for stable cell line development to Tempo Support. (Need a complete kit? Just ask us!)

Customized Reporter Cell Lines for TempoCal, TempoVol, TempoMito, TempoATP, or TempoO_2. Pairing Instructions: Step 1: Decide on a cell line. Step 2: Choose a Tempo Biosensor: TempoCal, TempoVol, TempoMito, TempoATP, or TempoO₂ Step 3: Need multiple biosensors per cell line? Step 4: Decide on the number of cells needed. Step 5: Email Tempo Support <support@tempobioscience.com>. Congratulations! You are done. Let us help you move your project forward! (Need a complete kit? Just ask us!)

TempoO₂™, a fluorescent biosensor, is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapid™. The biosensor assay offers fast kinetics (resolution: seconds to hours) in measuring real-time cellular metabolism and cytotoxicity. Analysis of cellular respiration directly reflects mitochondrial health and function. TempoO₂™-Rapid cells serve as a control for all products containing the TempoO₂™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest. For additional human cell lines and human cell types, please contact Tempo Support <support@tempobioscience.com>.

Academic scientists receive a special rate!

TempoATP™, a fluorescent biosensor, is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapidTM . The biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function.

TempoATP™-Rapid cells serve as a control for all products containing the TempoATP™ biosensor. To customize TempoRapid™ cells to scientists’ experimental needs, the cells are easily transfectable with gene(s)-of-interest.

For additional human cell lines and human cell types, please contact Tempo Support <support@tempobioscience.com>.

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TempoVol™-iCort: cationic voltage biosensor assay using human iPS-derived . They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction and maturation conditions. TempoVol™-iCort cells are polarized structures when plated as a monolayer in culture and express markers such as MAP2 and beta-Tuj. We've validated iCort™ cells to be cationic-conducting neurons when exposed to solutions with fluctuating concentrations of sodium, calcium, potassium, magnesium, and protons/H+. TempoVol™ biosensor is introduced to the cells using proprietary methods and is expressed in the in abundance in the cell.

Academic scientists receive a special rate!

TempoVol^TM cationic voltage biosensors are incorporated into human neural progenitor cells. They respond to intracellular ionic changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium/potassium/proton/sodium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoVol™ cationic voltage biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission: 610nm/625nm). It is incorporated into Tempo's proprietary immortalized human neuroepithelial cell line, TempoRapid^TM. TempoVol™ control cells serve as a control for all products containing the TempoVol™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoMito™ mitochondrial calcium biosensor responds as a fluorescent reporter: 605nm-625nm fluorescent range. It is incorporated into Tempo's proprietary immortalized human neuroepithelial cell line, TempoRapid^TM. TempoMito™ control cells serve as a control for all products containing the TempoMito™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoCal™ is a calcium biosensor that responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm) . It is incorporated into Tempo's proprietary immortalized human cell line, TempoRapid™. TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoMito™-iNStem cells comprise mitochondrial biosensors incorporated into human iPS-derived neural progenitor cells. Mitochondrial dysfunctions have been implicated in human neurological and psychiatric disorders, and neural progenitor cells have been used as a cellular model. Tempo's biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening.

Academic scientists receive a special rate!