Products

Tempo-iOsteo™ are reprogrammed using our pproprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology. They expression biomarkers: Osteocalcin, COL1A1, FLT1, Bone Sialoprotein, RUNX2, and SPP1 (q/RT-PCR data not shown). Bone mineralization data (Alizarin S Red; 530nm absorbance data) are shown.

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Please inquire for ready-to-ship Tempo-iOsteo™ lines. Regarding patients derived iOsteo cells, browse our TempoStemBank™-iPSC collection. 

Mesenchymal stem cells ("MSCs") are non-haematopoietic, multipotent multipotent stromal stem cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, myocytes, and adipocytes. MSCs express cell surface markers such as CD73, CD90, CD166, and CD105, in addition to Oct4 and Nanog. Tempo-iMSC™ are human iPSC-derived mesenchymal stem cells. NEW! For scientists working on exosomes, please contact us for conditional media for exosome extractions and biomarker validations. NEW! For scientists working on CD34+ HSCs, please email us regarding Tempo-iHSC^TM (SKU214; Custom Requests Only).

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Retinal pigment epithelium (RPE) is the pigmented cell layer surrounding the neurosensory retina. The cells support the retinal visual cells, and they are attached to the underlying choroid and overlying retinal visual cells. The RPE has several functions: light absorption, epithelial transport, spatial ion buffering, visual cycle, phagocytosis, secretion and immune modulation. Tempo-iRPE™ are human iPSC-derived retinal pigment epithelial cells. They express biomarkers such as RPE-65, RLBP1, and BEST.

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Tempo’s iMotorNer™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology conditions. Motor neurons have synapses that transmit via cholinergic transmitters. These synapses require extremely fast inactivations of its transmitter, acetylcholine (Ach), by utilizing the enzyme, acetylcholinesterase (AchT). AchT hydrolyzes acetylcholine to acetate and choline, as a way of inactivation. Tempo’s iMotorNer™ cells are polarized structures when plated as a monolayer in culture and express biomarkers, acetylcholinesterase (AchT), beta-TUJ (neuron-specific), Isl1 and HB9.

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Tempo’s iMG™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary feeder-free, serum-free, non-viral, nucleic-acids-free, integration-free reprogramming conditions. Tempo’s iMG™ microglia cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, A1F1(= IBA1), hGPR34, CXCR3 and CD11b/c. 

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TempoMito™ biosensor is an mitochondrial calcium flux sensor. The cells have been engineered to express TempoMito™ biosensor which has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Excitation=610nm; Emission=625nm (Filter-based fluorescent multiplate readers). TempoMito™ biosensor is introduced to the cells using proprietary magneto-liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoATP™, a fluorescent biosensor, is also custom-incorporated into the human TempoRapid™cell line . With TempoATP™ , the biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function. Excitation=535nm; Emission=550nm (Filter-based fluorescent multiplate readers).

TempoRapid™ cells are immortalized human neural-epithelial cells. The biosensors are transiently expressed. Please send inquiries for stable cell line development to Tempo Support. (Need a complete kit? Just ask us!)

Tempo-iKer™: human keratinocytes are derived from human dermal-fibroblast cells, using feeder-free, serum-free, genetic-elements-free, virus-free,  induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iKer™ cells are structures when plated as a monolayer in culture and express distinct biomarkers such as Cytokeratin-18. iKer™ can be used under maturation conditions, co-culture conditions (with fibroblasts, melanocytes or additional epidermal subtypes), and short-term functional characterization studies.

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Tempo-iMel™: human melanocytes are derived from human induced pluripotent stem cell (iPSC) lines under a fully defined proprietary reprogramming and maturation conditions. Tempo’s iMel™ cells are branched elaborate structures when plated as a monolayer in culture and express biomarkers such as Vimentin, S100beta, and cytokeratins. iMel™ can be used under maturation conditions, co-culture conditions (with fibroblasts, keratinocytes or additional epidermal subtypes), and short-term functional characterization studies. 

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TempoStemBank™-iPSC: human iPSC lines are derived from human dermal/fibroblasts, blood or cord blood, using our proprietary genetic/viral-elements-free, nucleic-acids-free, feeder-free, and serum-free reprogramming technology. The cells express canonical biomarkers of iPSCs (e.g., Oct4, Tra-1-60, Tra-1-81, Sox2, Nanog, and SSEA-4); they have been proven to be multipotent and are able to differentiate into ectoderm, endoderm and mesoderm derivative cell types. Please inquire for ready-to-ship low passage iPSCs (Free culture media included!). 

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If you would like to purchase them, please contact us to discuss your particular requirements. Please inquire for ready-to-ship Human iPSC-derived Hepatocytes. 96-well format. Ethnically diverse lines available.

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