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  1. TempoCal™-control cells image1
    TempoCal™-Rapid: Calcium Biosensor Assay using Immortalized Human Neuroepithelial Cells
    $595.00

    TempoCal™ is a calcium biosensor that responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm) . It is incorporated into Tempo's proprietary immortalized human cell line, TempoRapid™. TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

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  2. TempoMito responses2
    TempoMito™-iNStem: Mitochondrial Biosensor Assay using Human Neural Progenitors
    $950.00

    TempoMito™-iNStem cells comprise mitochondrial biosensors incorporated into human iPS-derived neural progenitor cells. Mitochondrial dysfunctions have been implicated in human neurological and psychiatric disorders, and neural progenitor cells have been used as a cellular model. Tempo's biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening.

    Academic scientists receive a special rate!

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  3. TempoCal™-iAstro; 1mM CaCl2
    TempoCal™-iAstro: Calcium Biosensor Assay using Human Astrocytes
    $950.00

    TempoCal™-iAstro: Tempo’s calcium biosensors are incorporated into induced human astrocytes. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm). With excellent signal-to-noise ratios, these biosensors operate on second-to-minute scales.

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  4. Immunocytochemistry with anti-COX IV antibodies
    TempoMito™-iAstro: Mitochondrial Biosensor Assay using Human Astrocytes
    $950.00

    TempoMito™-iAstro cells contain mitochondrial biosensors in human iPS-derived astrocytes. Disruption of mitochondrial functions in human astrocytes has been implicated in Alzheimer's disorder and ischemia. Tempo's biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening. 

    Academic scientists receive a special rate!

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  5. TempoCal with 20uM Histamine and 20uM Carbachol stimulations
    TempoCal™-iNStem: Calcium Biosensor Assay using Human Neural Progenitor Cells
    $950.00

    TempoCalTM calcium biosensors are incorporated into human neural progenitor cells. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

    Academic scientists receive a special rate!

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  6. iOligo (O4 marker, Green)
    Tempo-iOligo™: Human iPSC-derived Oligodendrocyte Progenitor Cells (OPCs)
    $940.00

    Oligodendrocytes belong to the family of glial cells in the human central and peripheral nervous system. They are multifunctional cells and are implicated in many disabling neurological disorders, including adult-onset demyelinating disorders, as well as leukodystrophies and cerebral palsy.

    Tempo’s iOligo™ are induced oligodendrocyte progenitors reprogrammed from human fibroblasts cells using a proprietary reprogramming technology. iOligo™ cells are cryopreserved at low passage numbers. Each vial contains ~1.05 x 10cells. iOligo™ cells are characterized via standard morphological examinations and via immunocytochemistry methods against known marker proteins (such as O4, PDGFalphaR, NG2, and CNPase) and can be induced to express MBP upon T3 hormone induction.

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    Academic scientists receive a special rate! Find new cell culture solutions here.

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  7. iAstro (TM) human astrocytes, light transmission
    Tempo-iAstro™: Human iPSC-derived Astrocytes
    $829.00

    Astrocytes, also known as Astroglia, belong to the family of glial cells in the human central and peripheral nervous system. They have been shown to have central roles in the maintenance of neuronal metabolism and neurotransmitter synthesis. They have been implicated in many human neurodegenerative disorders such as multiple sclerosis, amyotrophic lateral sclerosis (ALS), Alzheimer’s disorder, Huntington’s disease and Parkinson’s disease.

    Tempo’s iAstro™: human iPSC-derived astrocytes are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. Tempo’s iAstro cells are polarized structures when plated as a monolayer in culture and express astrocyte markers, GFAP, EAAT1, Aquaporin family genes, and S100beta, and they exhibit calcium wave activities (see figures shown above). There are two subtypes of human astrocytes: Type I and Type II. >90% of Tempo-iAstro™ astrocytes are Type II. If you need Type I astrocytes, please email Tempo Support.

    Check out our new citations! 

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  8. iNStem(TM) induced neural progenitor cells. Immunocytochemistry with anti-Vimentin antibodies.
    Tempo-iNStem™: Human iPSC-derived Neural Progenitor Cells
    $802.00

    Neural stem cells are self-renewing, multipotent cells that can generate neurons and glial-specific cellular lineages. Primarily, neural stem cells differentiate into neurons, astrocytes, and oligodendrocytes. Neural progenitor cells are adult stem cells committed to their neural and glial lineages.

    Tempo-iNStem™ cells are induced neural progenitor cells reprogrammed from human fibroblasts or blood cells using proprietary foot-print-free stem cell technologies. iNStem™ cells are cryopreserved at low passage numbers. Each vial will maintain 5 subsequent passages and expansions without spontaneous differentiations. Cells have been tested to be able to differentiate into multiple neural and glial subtypes. Each vial contains >1 x 10cells. iNStem™  cells are characterized via standard morphological examinations and via immunocytochemistry methods against known marker proteins (such as Vimentin).

    Academic scientists receive a special rate! Find new cell culture solutions here.

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  9. Cell Culture Solutions
    Cell Culture Solutions
    Our qualified cell culture media are specifically designed and optimized for your chosen cell types. Simply select your cell type and email us for cell culture solutions! Learn More
  10. Tempo-iSenso and Tempo-iSchwann co-culture, anti-MBP biomarker (ICC)
    Tempo-iSenso™: Human iPSC-derived Sensory Neurons
    $985.00

    Tempo-iSenso™ cells are human sensory neurons derived from human dermal cells, using human induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction and maturation conditions. Tempo’s iSenso™ cells are branched structures when plated as a monolayer in culture and express biomarkers such as beta-Tuj (neuronal specific), BRN3a and Peripherin. In addition, Tempo-iSenso™ cells express biomarkers such as ASIC, CNR1, NTRK, P2X4, SCN9a/Nav1.7, TRPA1, TRPV1, P2X3, and SCN10A/Nav1.8 (RT/qPCR data not shown).  iSenso™ can be used under maturation conditions or co-culture conditions (with Schwann cells or additional neuronal subtypes) for a variety of functional characterizations and drug candidate validation studies.  

    Academic scientists receive a special rate! Find new cell culture solutions here.

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