Category : iPSCs

Written on Jul, 08, 2020 by in ,
TempoATP – a snapshot

Genetically encoded biosensors have become popular and powerful LIVE-cell reporters in recent years (see reference here). These biosensors can be incorporated into a variety of human inducible pluripotent stem cells (iPSCs) and iPSC-derived cell types (such as neurons, glial cells, kidney cells, and cardiomyocytes, just to name a few). Previously, we discussed the biosensors and how they are used in research (here). TempoATP biosensor (image shown above) is a LIVE-cell reporter that tracks intracellular ATP levels in real time (from seconds to hours).

Examples of where mitochondrial ATP productions are important?

#1: Research from neurodegenerative disorders (e.g., Alzheimer’s, amyotrophic lateral sclerosis (ALS), Huntington’s, and Parkinson’s) has shown the importance of mitochondria in neuronal survival. In other words, ageing and neurodegeneration depend on mitochondrial health (see reference here).

#2: Cancer research has shown that mitochondrial production of ATP is associated with calcium homeostasis. Furthermore, tumorigenesis depends on ATP production and mitochondrial function (see reference here).

#3: Mitochondrial dysfunction and oxidative stress are key characteristics found in the ageing human skin (see reference here). Some of the notable phenotypes are: wrinkle formation, hair loss, uneven pigmentation, inflammation, photo-aging, and wound healing impairment (see reference here).

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Written on Aug, 07, 2019 by in , ,

Cardiomyocytes are cardiac muscle cells. They are terminally differentiated and facilitate contractile forces (“beatings”) of the heart. Grown in vitro as a monolayer sheath, cardiomyocytes are connected by gap junction proteins that help synchronize contraction-relaxation cycles of the cardiomyocytes. Cardiomyocytes may be used in various in vitro or in vivo studies; transplantation into normal or diseased systems; cardiac toxicology studies; or cardiovascular developmental studies. Cardiomyocytes have a high mitochondrial density, which allows them to produce adenosine triphosphate (ATP) quickly, making them highly resistant to fatigue.

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Written on Mar, 22, 2019 by in , ,

Welcome back to our cell of the month series. This time we’re talking about CD34+ cells, a type of undifferentiated multipotent hematopoetic stem cell (HSC) with the potential to differentiate into almost any other blood cell type under specific conditions. As stem cells, CD34+ cells naturally have the capacity for self-renewal, allowing them to divide and replicate indefinitely, making them a highly valuable source of hematopoetic cells in research and clinical settings. However, the CD34+ cell population in blood is extremely small, and is estimated to represent less than 0.5% of all other blood cell types.

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