Your tissue culture hood, cell culture hood, biosafety cabinet (or BSC), the hood-y thing in your tissue culture room or whatever you like to call it, is a precious tool that requires occasional love. Here we’ll teach you how to correctly clean your hood and reduce your lab’s risk of getting a “CONTAMINATED” sign stuck to your tissue culture room’s door.
As a child, I remember learning how to properly mop the kitchen floor. I don’t know who said it to me, but the advice I was given was:
“The floor should be so clean you can eat off it!”
This is great advice that makes you more of a perfectionist when it comes to cleanliness so I propose that the same be true for your tissue culture hood.
Now no one is suggesting you should enjoy your next quesadilla while splitting cells but innumerable researchers have lost months or even years of work because of contamination so keeping this idiom in mind could very well save you big-time down the road. Good aseptic technique is key to minimizing contamination but this can only go as far as everyone is the lab is thorough. You need a backup to further reduce risks: regularly deep cleaning your hood. Here we’re going to teach you how to do it.
This level of deep cleaning is best done every 6 months so keep a rotating scheduling and have people sign off when they did it. Pin these steps up in your tissue culture room. Before you start, make sure you know what type of hood you’re working with. These steps cover class II biological safety cabinets as they’re the most common type of hoods for tissue culture rooms but they can be applied to class I cabinets and fume hoods too (although you don’t need to be as meticulous). Lastly, any apparatus you’ll need for this job is highlighted in red so have these ready.Continue Reading...