Written on Aug, 26, 2022 by in

TempoATP™-Rapid: ATP Biosensor Assay, a fluorescent biosensor, was recently used in a study cited in nature.com. TempoATP™ offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. In this study scientists from the University of Salford and University of Pisa investigated whether human genes regulating ATP production in the mitochondria are important for breast cancer and tumor metastasis. In investigating ATP fluctuations in the living cell, researchers noted that ATP depletion is a consequence of mitochondrial dysfunction and triggers  cell death. Therefore, researchers explored the possibility that ATP depletion could be a solution to target aggressive cancer cells. Read on to learn more about this study’s conclusions and the usefulness of TempoATP™!

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Written on Aug, 26, 2020 by in , ,

“Fluidics” is the information extracted from bodily fluids and the understanding of liquids or fluids using small diameter volumes. Hippocrates (400 BC), Galen (200 AD) and Theophilus (700 AD) were interested in analyzing urine samples — to understand the human body and its functions (1). They compared and analyzed urine color, corresponding to patients’ symptoms. About 1000 years later, scientists worked on the behavior of fluids using glass containers with small volume and diameters.

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Written on Jul, 08, 2020 by in ,
TempoATP – a snapshot

Genetically encoded biosensors have become popular and powerful LIVE-cell reporters in recent years (see reference here). These biosensors can be incorporated into a variety of human inducible pluripotent stem cells (iPSCs) and iPSC-derived cell types (such as neurons, glial cells, kidney cells, and cardiomyocytes, just to name a few). Previously, we discussed the biosensors and how they are used in research (here). TempoATP biosensor (image shown above) is a LIVE-cell reporter that tracks intracellular ATP levels in real time (from seconds to hours).

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