TempoVol™-iNStem: Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells

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TempoVolTM cationic voltage biosensors are incorporated into human neural progenitor cells. They respond to intracellular ionic changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium/potassium/proton/sodium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoVol responses

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  • Tempo's iNStem, Vimentin biomarker (green)


TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission: 610nm/625nm) and is incorporated into Tempo's iPSC-derived neural progenitor cells, iNStem. iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoVol™ biosensor is introduced to the cells using proprietary liposome mediated methods.


  • Chemical compound or small molecules testing
  • Phenotypic Assays
  • Neurotoxicity testing for reporting cationic flux
  • Signal transduction pathway modulations
  • Ion Channel studies
  • Chemical compound or small molecules testing or screening
  • For low, medium, or high-throughput applications

Specifications: ~2x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

QC: Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.


Additional Information

Product Use TempoVol™-iNStem are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.