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TempoCal™-Rapid: Calcium Biosensor Assay using Immortalized Human Neuroepithelial Cells

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Summary

TempoCal™ is a calcium biosensor that responds as a fluorescent reporter: 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm) . It is incorporated into Tempo's proprietary immortalized human cell line, TempoRapid™. TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor. This product can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoCal™-control cells image1

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Details

Tempo's calcium biosensor responds in the 605nm-625nm fluorescent range (Excitation/Emission) and is incorporated into Tempo's proprietary immortalized human cell line, TempoRapid™. TempoCal™-control cells serve as a control for all products containing the TempoCal™ biosensor.

Applications:

  • Chemical compound or small molecules testing
  • Phenotypic Assays
  • Control cell line with biosensor incorporated (e.g., establishing baseline response levels, testing expression constructs)
  • For single-cell applications, as well as medium to high-throughput screening platforms.

Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Starting Materials: immortalized human cell line of neuro-epithelial origin. Cells should grow fairly well (doubling time <36 h) without passage limitation in a conventional medium DMEM or DMEM/F12 media with a maximum of 10% serum and selection antibiotics (G418, hygromycin or puromycin) at reasonable concentrations. 

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU108

Additional Information

Product Use TempoCal™-control cells are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.