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TempoMito™-U2OS

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$495.00
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Summary

U2-OS cell line, originally known as the 2T cell line, is an immortalized human cancer cell line. It is widely used in research and drug development, as it offers a variety of application potentials. TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). U2-OS stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. 


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Details

U2-OS cell line, originally known as the 2T cell line, is an immortalized human cancer cell line. It is widely used in research and drug development, as it offers a variety of application potentials.

TempoMito™ biosensor is an mitochondrial calcium flux sensor. It is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV). U2-OS stably expressing TempoMito™ biosensor has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter.

Culture Medium: McCoy′s 5a medium with 1.5 mM Glutamine or DMEM with 2mM L-Glutamine; 10% Fetal Bovine Serum (FBS). Subculture Routine: Split sub-confluent using 0.025% trypsin/EDTA solution. Allow the flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. Passage ratio: 1-to-6.

Applications:

  • Cellular toxicity testing for reporting mitochondrial calcium fluctuations
  • Phenotypic Assays (i.e., mitochondrial toxicity and function)
  • Chemical compound or small molecules testing
  • Cell morphology or cell death research studies.
  • High Content Assays
  • Live Cell Imaging
  • Receptor studies (e.g., IGF-I, IGF-II)
  • Mitochondrial metabolism 
  • Cellular metabolism 

QC:

Sterility

Safety 

HIV/viruses, bacteria, fungi: negative

Cell viability post-thawing (>90%)

1 vial = ~0.5x10^6 cells

SKU301

Prefer a custom cell line product? Contact <support@tempobioscience.com>

Cell Line Name   Histology Type
U2OS   Bone carcinoma
HEK293   Immortalized Kidney Epithelial
SW 480   Colorectal adenocarcinoma
U87MG   Brain cancer
Hep G2   Hepatocellular carcinoma
NIH3T3   Embroyonic fibroblast, murine
HT1080   Carcinoma (connective tissue)
HCT116   Colorectal cancer
MCF 7   Breast cancer
SW 1990   Pancreatic cancer
NCI-H2126   Non-Small cell cancer
HUVEC   Umbilical vascular endothelium
HeLa   Cervical epithelial adenocarcinoma
CG4   Glial cell line (from rat)
A-204   Muscle epithelial
A549   Lung epithelial cell line
BT-142   Oligoastrocytoma, suspension
NK-92   Peripheral blood, natural killer cells
CHO   Chinese hamster ovarian cell line
NCI-H69   Lung tumor cell aggregate, suspension
ARPE-19   Retinal pigmented epithelial
Beta-TC-6   Beta cell (mouse)
BxPC-3   Pancreatic epithelial, adenocarcinoma
Jurkat cells   Peripheral T lymphocyte, suspension
SW 780   Urinary bladder epithelial carcinoma
MeT-5A   Mesothelium, from non-cancerous
Mesenchymal stromal cells   email <support@tempobioscience.com>
Chemokine Reporter cells   email <support@tempobioscience.com>
cell line of your choice   email <support@tempobioscience.com>

References:

Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y. Toxicol Sci. 2008 Jun;103(2):335-45. 

Dykens JA, Will Y. Drug Discov today. 2007 Sep; 12 (17-18): 777-85.

 

Additional Information

Product Use TempoMito™-U2OS are intended for basic scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.