TempoCal™-iAstro: Calcium Biosensor Assay using Human Astrocytes

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$950.00
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Summary

TempoCal™-iAstro: Tempo’s calcium biosensors are incorporated into induced human astrocytes. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission=610nm/625nm). With excellent signal-to-noise ratios, these biosensors operate on second-to-minute scales.

TempoCal™-iAstro; 1mM CaCl2

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  • TempoCal™-iAstro: stimulated with 20uM Histamine
  • TempoCal™-iAstro: quantified with stimulation
  • TempoCal™-iAstro: stimulated with 20uM Histamine

Details

Tempo’s calcium biosensors are incorporated into human iPS-derived astrocytes. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission), measuring fluctuations in cytosolic calcium concentrations. "Calcium Wave" is a key feature of functional human astrocytes. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput assay platforms.

Applications:

  • Neurotoxicity testing for reporting cellular calcium fluctuations
  • Chemical compound or small molecules testing
  • Research and Development on myelin and neurodegenerative disorders 
  • Studying astrocyte disorders, astrocyte differentiation and function, and calcium-mediated signal transduction pathways.

Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU106

Additional Information

Product Use TempoCal™-iAstro are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.