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Tempo-iOsteo™: Human iPS-derived Osteoblasts

Summary

Tempo-iOsteo™ are reprogrammed using our proprietary non-viral/genetic, foot-print-free technology. They expression biomarkers: Osteocalcin, COL1A1, FLT1, Bone Sialoprotein, RUNX2, and SPP1 (q/RT-PCR data not shown). Bone mineralization data (Alizarin S Red; 530nm absorbance data) are shown.


Please inquire for ready-to-ship Tempo-iOsteo™ linesRegarding patients derived iOsteo cells, browse our TempoStemBank™-iPSC collection. 

Tempo-iOsteo, healthy donor, Alizarin S Red assay

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  • Tempo-iOsteo, Alizarin S Red assay
  • Tempo-iOsteo, healthy donor, Alizarin S Red assay
  • Tempo-iOsteo as a group of connected cells
  • Tempo-iOsteo, healthy donor, Alizarin S Red assay

Details

Tempo-iOsteo™ cells are human iPS-derived osteoblasts. Osteoblasts are specialized cell types derived from the mesenchymal stem cells lineage. They contribute to human bone formation. Osteoblasts synthesize collagen and express specialized proteins such as osteoclacin and osteopontin. As an organized group of connected cells, osteoblasts form part of the mineralized matrix. The matrix is part of the crucial skeletal support for humans. Mineralization is part of human's physiological homeostasis, such as acid-base balance, calcium or potassium maintenance. The iOsteo cells serve as a model system to examine bone mineralization, matrix formation for skeletal systems, bone remodeling, and bone metabolism.  The iOsteo models can be used to uncover the molecular mechanisms underlying bone loss, osteoporosis, osteoclast inhibition, rheumatoid arthritis, trauma, and bone matrix formation.

Tempo-iOsteo™ are reprogrammed using our proprietary non-viral/genetic, foot-print-free technology. They expression biomarkers: Osteocalcin, COL1A1, FLT1, Bone Sialoprotein, RUNX2, and SPP1 (q/RT-PCR data not shown). Bone mineralization data (Alizarin S Red; 530nm absorbance data) are shown.

Please inquire for ready-to-ship Tempo-iOsteo™ lines. Regarding patients derived iOsteo cells, browse our TempoStemBank™-iPSC collection. 

Applications:

  • Inflammatory biomarkers (e.g., cytokines inductions)
  • Signaling pathways (e.g., growth factors, cytokines, and interleukins)
  • Bone formation, morphogenesis, metabolism, and differentiation
  • 3D cell-based bone models
  • Biomarkers from bone cancers (e.g., osteosarcoma)

Patient specific derivation of human iPS-derived osteoblasts:

Characterizations performed: biomarker confirmations; Alizarin S Red assay; functional secretion assays (upon request).

Approximate turn-around time: ~14-16 weeks (from fibroblasts or blood) and ~6-9 weeks (for iPSCs)

Sample requirements: human fibroblasts or iPSCs, ~5x10^5 cells. Please contact us, if you'd prefer to use blood samples

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

Deliverables: >2x10^6 cells (scale-up possible). BioSafety Level 2.

SKU 1012

Additional Information

Product Use Tempo-iOsteo™ are intended for basic scientific research, drug discovery, and therapeutics development use only. It is not a product for human testing, therapeutics, or diagnostics.