new

Tempo-iCardio™: Human iPSC-derived Cardiomyocytes

Summary

Tempo-iCardio™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines using a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.  They are cultured as monolayers and are alive for >4 weeks under defined cell culture conditions. Tempo-iCardio™ cells express biomarkers: ACTC1, CSPR3, KCNQ1, MYBPC3, MYH7, MYL2, PKP2, PRKAG2, TNNI3, TNNT, TPMI, TTN. Immunocytochemistry data show the expressions of TNNT2 and E-Cadherin.

Tempo-iCardio™ anti-TNNT2 antibody (ThermoFisher)

More Views

  • Tempo-iCardio™ anti-E-Cadherin antibody (CST)
  • Tempo-iCardio™ mRNA analysis

Details

Cardiomyocytes are cardiac muscle cells. They are terminally differentiated and facilitate contractile forces ("beatings") of the human heart. Grown in vitro as a monolayer, cardiomyocytes are connected by gap junction proteins that help synchronize contraction-relaxation cycles of the cardiomyocytes. Cardiomyocytes may be used in various in vitro or in vivo studies: 1) diseased modeling; 2) cardiac toxicology studies; or 3) cardiovascular developmental studies. Cardiomyocytes have very high mitochondrial density, which allows them to produce adenosine triphosphate (ATP) at high levels in order to sustain cellular functions.

Tempo-iCardio™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines using a fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.  They are cultured as monolayers and are alive for >4 weeks under defined cell culture conditions. Tempo-iCardio™ cells express biomarkers: ACTC1, CSPR3, KCNQ1, MYBPC3, MYH7, MYL2, PKP2, PRKAG2, TNNI3, TNNT, TPMI, TTN (mRNA data shown above). Immunocytochemistry show the expressions of TNNT2 and E-Cadherin.

Applications:

 • Cardiac muscle disease modeling (e.g., Fabry disease, primary arrhythmias, cardiomyopathies, Pompe disease)

 • Cardiac toxicity and safety modeling (e.g., calcium flux assays, hERG)

 • Metabolic studies (e.g., ATP assays, mitochondrial assays)

 • Phenotypic Screening (e.g., ion channel studies, Na/K/Ca inhibitor assays)

Academic scientists receive a special rate! Please inquire for ready-to-ship Tempo-iCardio™ lines. Browse our TempoStemBank™-iPSC collection.

Technology used: an in-house developed proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.

Specifications: ~1.0x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU1014

 

Additional Information

Product Use Tempo-iCardio™ are intended for scientific research, drug discovery, and therapeutics development use only. It is not a product for human testing, therapeutics, or diagnostics.