TempoVol™-Rapid: Cationic Voltage Biosensor Assay using Immortalized Human Neuroepithelial Cells

TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission: 610nm/625nm) and is incorporated into Tempo's proprietary immortalized human neuro-epithelial cell line, TempoRapid^TM. TempoVol™-Rapid cells serve as a control for all products containing the TempoVol™ biosensor. 

Applications:

• Chemical compound or small molecules testing
• Phenotypic Assays
• Control cell line with biosensor incorporated (e.g., establishing baseline response levels, testing expression constructs)
• For low, medium, or high-throughput applications

Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Starting Materials: immortalized human cell line of neuro-epithelial origin. Cells should grow fairly well (doubling time < 36 h) without passage limitation in a conventional medium: DMEM or DMEM/F12 media with a maximum of 10% serum, 1x essential amino acids, Pen/Strep and selection antibiotics (such as G418, hygromycin or puromycin) at experimentally determined concentrations for each user’s experiment if a gene-of-interest is introduced with a selection marker.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

SKU120