TempoDual™-ATPMito-Rapid: Dual Biosensor Cytotoxicity Assay

TempoMito™ biosensor is an mitochondrial calcium flux sensor. The cells have been engineered to express TempoMito™ biosensor which has been developed for monitoring mitochondrial calcium flux and cytotoxicity using a highly sensitive fluorescent based biosensor as reporter. Excitation=610nm; Emission=625nm (Filter-based fluorescent multiplate readers). TempoMito™ biosensor is introduced to the cells using proprietary magneto-liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

TempoATP™, a fluorescent biosensor, is also custom-incorporated into the human TempoRapid™ cell line . With TempoATP™ , the biosensor assay offers fast kinetics (resolution is in seconds) in measuring real-time cellular ATP metabolism and cytotoxicity. Analysis of cellular oxidative phosphorylation directly reflects mitochondrial health and function. Excitation=535nm; Emission=550nm (Filter-based fluorescent multiplate readers).

TempoRapid™ cells are immortalized human neural-epithelial cells. The biosensors are transiently expressed with the biosensors. Please send inquiries for stable cell line development to Tempo Support. Alternative cell lines are available. Please see Frequently Requested Cell Lines below.

Applications:

• Cytotoxicity Assays

• Chemical compound or small molecules screening

• Cellular Metabolism Assays

• Mitochondrial  metabolism research

• Live cell imaging (time-course studies and drug treatments).

• Cellular toxicity testing for reporting mitochondrial calcium fluctuations

• Phenotypic Assays (i.e., mitochondrial toxicity and function)

• Chemical compound or small molecules testing

• Cell morphology or cell death research studies.

Academic scientists receive a special rate!

Specifications: ~0.5x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Starting Materials: immortalized human cell line. Cells grow fairly well (doubling time ~72hrs) without passage limitation in a conventional medium: DMEM with a maximum of 10% serum, 1x Essential amino acids, Pen/Strep and selection antibiotics (such as G418, hygromycin or puromycin) at experimentally determined concentrations for each user’s experiment if a gene-of-interest is introduced with a selection marker. Culture Medium: DMEM with 2mM L-Glutamine; 10% Fetal Bovine Serum (FBS). Subculture Routine: Split sub-confluent using 0.025% trypsin/EDTA solution. Allow the flask to sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. Passage ratio: 1-to-6.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC:
Sterility, Safety (BioSafety Level 2)
HIV/viruses, bacteria, fungi: negative
Cell viability post-thawing (>90%)

SKU403

Frequently Requested Cell LInes: