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TempoMito™-iNStem: Mitochondrial Biosensor Assay using Human Neural Progenitors

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$950.00
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Summary

TempoMito™-iNStem cells comprise mitochondrial biosensors incorporated into human iPS-derived neural progenitor cells. Mitochondrial dysfunctions have been implicated in human neurological and psychiatric disorders, and neural progenitor cells have been used as a cellular model. Tempo's biosensor responds to mitochondrial calcium changes and reports in the 605nm-625nm fluorescent range (Excitation/Emission) as calcium levels increase or decrease. With excellent signal-to-noise ratios, these sensors can operate on second-to-minute scales. This tool provides an efficient way of detecting changes in the mitochondrial organelle and enables high-throughput screening approaches for drug discovery and toxicology screening.

TempoMito responses2

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  • TempoMito-iNStem
  • TempoMito responses
  • TempoMito 2.5mM CaCl2
  • TempoMito responses3
  • Neural Progenitor cells, ICC, Vimentin marker

Details

TempoMito™-iNStem;: Mitochondrial Calcium Biosensor Assay using human Neural Stem Cells. They are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary neural induction condition. TempoMito™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoMito biosensor is introduced to the cells using proprietary liposome mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

Applications:

  • Neurotoxicity testing for reporting cellular calcium fluctuations
  • Phenotypic Assays (i.e., mitochondrial toxicity and function)
  • Chemical compound or small molecules testing
  • Research and development on animal models for human diseases
  • Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction pathways.

Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU107

Reference(s):
Martin LJ. Prog Mol Biol Transl Sci. 2012;107:355-415.
Martin LJ. Pharmaceuticals (Basel). 2010;3(4):839-915.
Chen K, Northington FJ, Martin LJ. Brain Struct Funct. 2010 Mar;214(2-3):219-34.
Palomo GM, Manfredi G. Brain Res. 2015 May 14;1607:36-46.

Additional Information

Product Use

TempoCal™-iNStem cells are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.