TempoCal™-iNStem: Calcium Biosensor Assay using Human Neural Progenitor Cells

Availability: In stock

$950.00
OR

Summary

TempoCalTM calcium biosensors are incorporated into human neural progenitor cells. They respond to intracellular calcium changes in the 605nm-625nm fluorescent range (Excitation/Emission = 610nm/625nm), measuring increases or decreases in cytosolic calcium concentrations. With excellent signal-to-noise ratios, these calcium biosensors can operate on second-to-minute scales. They can be used for single-cell applications, as well as medium to high-throughput screening platforms.

TempoCal with 20uM Histamine and 20uM Carbachol stimulations

More Views

  • TempoCal-iNStem™
  • TempoCal-iNStem™
  • Kinetic Responses of TempoCal in HEK293t Cells

Details

TempoCal™-iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro reprogramming conditions. TempoCal™ biosensor is introduced to the cells using proprietary liposome mediated methods.

Applications:

  • Neurotoxicity testing for reporting cellular calcium fluctuations
  • Chemical compound or small molecules testing or screening
  • Research and Development on animal models
  • Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction pathways.

Specifications: ~1x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU104

Additional Information

Product Use TempoCal™-iNStem are intended for basic scientific research and therapeutics development use only. Not for use in human testing or diagnostic procedures.