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Tempo-iMG™: Human iPS-derived Microglia (Ready-to-Ship vials)

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Summary

Tempo-iMG™: Human iPS-derived Microglia (Ready-to-Ship vials)
Tempo’s iMG™ cells are reprogrammed from human iPS-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, genetic-elements-free, and integration-free conditions. Tempo’s iMG™ cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, hGPR34, CXCR3 and CD11b/c. Academic scientists receive a special rate!

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Details

Tempo-iMG™ cells are reprogrammed from human iPS-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, genetic-elements-free, and integration-free conditions. Tempo’s iMG™ cells are polarized structures when plated as a monolayer in culture and express microglia biomarkers, such as ApoE/variants, hP2RY-12, TREM2, hGPR34, CXCR3 and CD11b/c. They exhibit phagocytosis activities (shown via LIVE-cell phagocytosis assay using fluorescent beads). Microglia are different from macrophages in many siginificant ways: synaptic stripping, secreting cytotoxic molecules, removing waste molecules from neurons, and signaling robust calcium wave responses.

Applications:

  • Disease modeling
  • Cytotoxicity evaluations
  • Phenotypic Screening (e.g., cytokine secretion, expression of differentiation markers, phagocytosis assays, synaptic denervations)
  • In vitro assays using microglial cells

Academic scientists receive a special rate!

Technology used: an in-house developed proprietary feeder-free, serum-free, viral/genetic-elements-free, integration-free reprogramming method.

Specifications: ~1.0x10^6 cells per 1ml of freezing medium (vial)

Long-term Storage: liquid nitrogen

Growth Properties: adherent

Storage: remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

QC: Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>90%)

SKU1001.1

Additional Information

Product Use Tempo-iMG™ are intended for basic scientific research, drug discovery, and therapeutics development use only. It is not a product for human testing or diagnostics.